{"title":"A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation.","authors":"Hervé Seitz, Jogender S Tushir, Phillip D Zamore","doi":"10.1186/1758-907X-2-4","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>MicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U).</p><p><strong>Results: </strong>Our analyses of fly, worm and mouse miRNA sequence data reveal that the 5'-U is recognized after miRNA production. Only one of the two strands can be assembled into Argonaute protein from a single miRNA/miRNA* molecule: in fly embryo lysate, a 5'-U promotes miRNA loading while decreasing the loading of the miRNA*.</p><p><strong>Conclusion: </strong>We suggest that recognition of the 5'-U enhances Argonaute loading by a mechanism distinct from its contribution to weakening base pairing at the 5'-end of the prospective miRNA and, as recently proposed in Arabidopsis and in humans, that it improves miRNA precision by excluding incorrectly processed molecules bearing other 5'-nt.</p>","PeriodicalId":88703,"journal":{"name":"Silence","volume":"2 ","pages":"4"},"PeriodicalIF":0.0000,"publicationDate":"2011-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/1758-907X-2-4","citationCount":"56","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Silence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/1758-907X-2-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 56
Abstract
Background: MicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U).
Results: Our analyses of fly, worm and mouse miRNA sequence data reveal that the 5'-U is recognized after miRNA production. Only one of the two strands can be assembled into Argonaute protein from a single miRNA/miRNA* molecule: in fly embryo lysate, a 5'-U promotes miRNA loading while decreasing the loading of the miRNA*.
Conclusion: We suggest that recognition of the 5'-U enhances Argonaute loading by a mechanism distinct from its contribution to weakening base pairing at the 5'-end of the prospective miRNA and, as recently proposed in Arabidopsis and in humans, that it improves miRNA precision by excluding incorrectly processed molecules bearing other 5'-nt.
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