{"title":"Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs.","authors":"M Dilipkumar, M Rajasimman, N Rajamohan","doi":"10.4061/2011/708043","DOIUrl":null,"url":null,"abstract":"<p><p>Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH(4)NO(3)-6.63 mg/gds, MnSO(4)·7H(2)O-26.16 mg/gds, Soya bean cake-60.6 mg/gds, and K(2)HPO(4)-5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.</p>","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2011 ","pages":"708043"},"PeriodicalIF":0.0000,"publicationDate":"2011-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085327/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Research International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4061/2011/708043","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH(4)NO(3)-6.63 mg/gds, MnSO(4)·7H(2)O-26.16 mg/gds, Soya bean cake-60.6 mg/gds, and K(2)HPO(4)-5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.
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