Whole-mount in situ hybridization in amphimedon.

CSH protocols Pub Date : 2008-12-01 DOI:10.1101/pdb.prot5096
Claire Larroux, Bryony Fahey, Maja Adamska, Gemma S Richards, Marie Gauthier, Kathryn Green, Erica Lovas, Bernard M Degnan
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引用次数: 21

Abstract

INTRODUCTIONDevelopmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.

两栖动物全载原位杂交。
发育基因表达主要通过使用地高辛标记的RNA探针进行全贴装原位杂交分析。本方案描述了如何在昆士兰Amphimedon中进行这一过程,包括固定、杂交和胚胎、幼虫和幼虫后幼鱼阶段的切片。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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