Establishing and maintaining a colony of planarians.

CSH protocols Pub Date : 2008-10-01 DOI:10.1101/pdb.prot5053
Néstor J Oviedo, Cindy L Nicolas, Dany S Adams, Michael Levin
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引用次数: 61

Abstract

INTRODUCTIONTo provide sufficient material for experimentation, a laboratory needs to expand and maintain a colony of planarians. It is crucial to keep a stable, healthy population of animals in a consistent environment to avoid inter-animal variability and modifier effects that can mask true phenotypes from experimental perturbation. In this protocol, we describe basic procedures for establishing and maintaining healthy colonies of Dugesia japonica, Schmidtea mediterranea, and Girardia tigrina (commonly found in the wild and commercially available in the United States). Although the recommendations are based on our optimization of conditions for G. tigrina, many of the procedures (such as food preparation and feeding strategy) can be applied to other species. For best results, the culture water must be carefully monitored and adjusted for each species.

建立和维持一个涡虫群落。
引言为了为实验提供足够的材料,实验室需要扩大和维持涡虫群落。至关重要的是,要在一致的环境中保持稳定、健康的动物种群,以避免动物间的变异性和修饰效应,从而掩盖实验干扰的真实表型。在本方案中,我们描述了建立和维持日本杜氏菌、地中海Schmidtea和虎吉拉氏菌(常见于野外,在美国可买到)健康菌落的基本程序。尽管这些建议是基于我们对虎蛛条件的优化,但许多程序(如食物制备和喂养策略)也可以应用于其他物种。为了获得最佳效果,必须仔细监测并调整每个物种的培养水。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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