Distribution of virulence genes in spa types of methicillin-resistant Staphylococcus aureus isolated from patients in intensive care units.

Korean Journal of Laboratory Medicine Pub Date : 2011-01-01 DOI:10.3343/kjlm.2011.31.1.30

Taeksoo Kim, Jongyoun Yi, Ki Ho Hong, Jeong-Su Park, Eui-Chong Kim

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引用次数: 26

Abstract

Background: Various virulence factors and superantigens are encoded by mobile genetic elements. The relationship between clonal background and virulence factors differs in different geographic regions. We compared the distribution and relationship of spa types and virulence genes among methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from a tertiary hospital in 2000-01 and 2007-08.

Methods: In 2000-01 and 2007-08, 94 MRSA strains were collected from 3 intensive care units at a Korean tertiary hospital. We performed spa typing and multiplex PCR for 19 superantigen genes.

Results: Relatively frequent spa types were t037 (40.5%), t002, t601, and t2138 in 2000-01, and t2460 (43.9%), t002, t037, t601, t324, and t2139 in 2007-08. We identified 4 novel spa types, 2 of which were designated as t5076 and t5079. Superantigen profiles were closely linked to spa types. For example, sea, sek, and seq superantigen genes were mainly detected in t037 strains.

Conclusions: Major spa types differed depending on study periods, and the distribution of superantigen genes correlated with spa type.

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重症监护病房患者分离的spa型耐甲氧西林金黄色葡萄球菌毒力基因分布

背景:各种毒力因子和超抗原都是由可移动的遗传元件编码的。不同地理区域克隆背景与毒力因子的关系不同。比较某三级医院2000-01年度和2007-08年度耐甲氧西林金黄色葡萄球菌(MRSA)菌株spa型和毒力基因的分布及关系。方法:2000-01年和2007-08年在韩国某三级医院3个重症监护病房采集MRSA菌株94株。对19个超抗原基因进行spa分型和多重PCR检测。结果:2000-01年度比较常见的spa类型为t037(40.5%)、t002、t601、t2138; 2007-08年度比较常见的spa类型为t2460(43.9%)、t002、t037、t601、t324、t2139。我们发现了4种新的spa类型，其中2种被命名为t5076和t5079。超抗原谱与spa类型密切相关。例如，在t037株中主要检测到sea、sek和seq超抗原基因。结论:主要温泉类型随研究时间的不同而不同，超抗原基因的分布与温泉类型相关。

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来源期刊

Korean Journal of Laboratory Medicine 医学-医学实验技术

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