A Comparison of the Dynamics of S100B, S100A1, and S100A6 mRNA Expression in Hippocampal CA1 Area of Rats during Long-Term Potentiation and after Low-Frequency Stimulation.

Cardiovascular psychiatry and neurology Pub Date : 2010-01-01 Epub Date: 2010-08-30 DOI:10.1155/2010/720958

Pavel D Lisachev, Mark B Shtark, Olga O Sokolova, Vladimir O Pustylnyak, Mary Yu Salakhutdinova, Oleg I Epstein

{"title":"A Comparison of the Dynamics of S100B, S100A1, and S100A6 mRNA Expression in Hippocampal CA1 Area of Rats during Long-Term Potentiation and after Low-Frequency Stimulation.","authors":"Pavel D Lisachev, Mark B Shtark, Olga O Sokolova, Vladimir O Pustylnyak, Mary Yu Salakhutdinova, Oleg I Epstein","doi":"10.1155/2010/720958","DOIUrl":null,"url":null,"abstract":"The interest in tissue- and cell-specific S100 proteins physiological roles in the brain remains high. However, necessary experimental data for the assessment of their dynamics in one of the most important brain activities, its plasticity, is not sufficient. We studied the expression of S100B, S100A1, and S100A6 mRNA in the subfield CA1 of rat hippocampal slices after tetanic and low-frequency stimulation by real-time PCR. Within 30 min after tetanization, a 2–4 fold increase of the S100B mRNA level was observed as compared to the control (intact slices) or to low-frequency stimulation. Subsequently, the S100B mRNA content gradually returned to baseline. The amount of S100A1 mRNA gradually increased during first hour and maintained at the achieved level in the course of second hour after tetanization. The level of S100A6 mRNA did not change following tetanization or low-frequency stimulation.","PeriodicalId":88441,"journal":{"name":"Cardiovascular psychiatry and neurology","volume":"2010 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2010/720958","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cardiovascular psychiatry and neurology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2010/720958","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2010/8/30 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 21

Abstract

The interest in tissue- and cell-specific S100 proteins physiological roles in the brain remains high. However, necessary experimental data for the assessment of their dynamics in one of the most important brain activities, its plasticity, is not sufficient. We studied the expression of S100B, S100A1, and S100A6 mRNA in the subfield CA1 of rat hippocampal slices after tetanic and low-frequency stimulation by real-time PCR. Within 30 min after tetanization, a 2–4 fold increase of the S100B mRNA level was observed as compared to the control (intact slices) or to low-frequency stimulation. Subsequently, the S100B mRNA content gradually returned to baseline. The amount of S100A1 mRNA gradually increased during first hour and maintained at the achieved level in the course of second hour after tetanization. The level of S100A6 mRNA did not change following tetanization or low-frequency stimulation.

Abstract Image

查看原文本刊更多论文

长期增强与低频刺激后大鼠海马CA1区S100B、S100A1、S100A6 mRNA表达的动态比较

对组织和细胞特异性S100蛋白在大脑中的生理作用的兴趣仍然很高。然而，必要的实验数据来评估他们的动态在最重要的大脑活动之一，它的可塑性，是不够的。采用实时荧光定量PCR方法研究强直和低频刺激后大鼠海马切片CA1亚区S100B、S100A1和S100A6 mRNA的表达。破伤风后30分钟内，与对照组(完整切片)或低频刺激相比，观察到S100B mRNA水平增加2-4倍。随后，S100B mRNA含量逐渐恢复到基线水平。S100A1 mRNA的表达量在破伤风后第1小时逐渐增加，并在破伤风后第2小时维持在达到的水平。在破伤风和低频刺激后，S100A6 mRNA水平没有变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cardiovascular psychiatry and neurology

自引率

0.00%

发文量