[Optimization of method of titration rabies virus with fluorescence focus units assay].

Abstract

Objective: A new Fluorescence Focus Units (FFU) developed for Rabies Virus titration replace current reference method (LD50) so as to reduce the cost.

Methods: The BSR cell was infected by triple serial dilution of the Rabies Virus CTN strain, then detect the fluorescent focus by FITC labeling anti-Rabies Virus monoclonal antibodies. All the samples were assayed in both FFU and LD50.

Results: Data generated indicateed that a significant correlation between the two methods (r=99).

Conclusion: This method is simple and rapid. It will be considered as a useful alternative method to the LD50.