Functionalization of iron oxide nanoparticles with a versatile epoxy amine linker.

Michael Nickels, Jingping Xie, Jared Cobb, John C Gore, Wellington Pham
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引用次数: 19

Abstract

A synthetically diverse linker molecule consisting of both a terminal epoxide and a terminal amine has been synthesized and shown to have the desired reactivity. Proof of principle experimentation showed that the prepared linker molecule possessed the ability to be reactive towards dextran coated iron nanoparticles, essentially converting the surface alcohols to amines with an efficiency on average of 50 linkers per nanoparticle. Once the surface of the nanoparticles had been functionalized, the iron nanoparticles were subsequently functionalized with both folic acid and fluorescein isothiocyanate, with an average efficiency of 20 and 3 molecules per nanoparticle, respectively. The labeled nanoparticles were then incubated with both folate receptor positive and negative cell lines, which showed a preferential accumulation of the particles in the receptor positive cell line. In addition to the fluorescence based assays, accumulation of the nanoparticles was demonstrated using T2-weighted MRI imaging, which showed that the iron core of the nanoparticle was present within the desired cell line. Overall, this linker has shown the ability to functionalize the surface of nanoparticles and can theoretically be used to label a wide variety of other targeting agents or imaging agents for in vivo therapies or diagnostics.

Abstract Image

Abstract Image

Abstract Image

氧化铁纳米颗粒与多功能环氧胺连接剂的功能化。
合成了一种由末端环氧化物和末端胺组成的合成多样连接分子,并显示出所需的反应活性。原理证明实验表明,所制备的连接剂分子具有对右旋糖酐包覆的铁纳米颗粒反应的能力,基本上以平均每个纳米颗粒50个连接剂的效率将表面醇转化为胺。一旦纳米颗粒的表面被功能化,铁纳米颗粒随后被叶酸和异硫氰酸荧光素功能化,平均效率分别为每纳米颗粒20和3个分子。然后将标记的纳米颗粒与叶酸受体阳性和阴性细胞系孵育,结果表明,叶酸受体阳性细胞系中的纳米颗粒优先积累。除了基于荧光的检测外,利用t2加权MRI成像证实了纳米颗粒的积累,这表明纳米颗粒的铁核存在于所需的细胞系中。总的来说,这种连接体已经显示出纳米颗粒表面功能化的能力,理论上可以用于标记各种其他靶向剂或显像剂,用于体内治疗或诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Materials Chemistry
Journal of Materials Chemistry 工程技术-材料科学:综合
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1.5 months
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