Alessandra Luchini, Caterina Longo, Virginia Espina, Emanuel F Petricoin, Lance A Liotta
{"title":"Nanoparticle technology: Addressing the fundamental roadblocks to protein biomarker discovery.","authors":"Alessandra Luchini, Caterina Longo, Virginia Espina, Emanuel F Petricoin, Lance A Liotta","doi":"10.1039/b822264a","DOIUrl":null,"url":null,"abstract":"<p><p>The incorporation of affinity baits into N-isopropylacrylamide-hydrogel-based nanoparticles offers a novel technology that addresses the major analytical challenges of disease biomarker discovery. In solution in complex biologic fluids (e.g. blood or urine), core-shell bait-containing nanoparticles can perform three functions in one step: (a) sieve molecules according to size, (b) sequestrate and concentrate target analytes, and (c) protect analytes from degradation.</p>","PeriodicalId":16297,"journal":{"name":"Journal of Materials Chemistry","volume":"19 29","pages":"5071-5077"},"PeriodicalIF":0.0000,"publicationDate":"2009-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888266/pdf/nihms200084.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/b822264a","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The incorporation of affinity baits into N-isopropylacrylamide-hydrogel-based nanoparticles offers a novel technology that addresses the major analytical challenges of disease biomarker discovery. In solution in complex biologic fluids (e.g. blood or urine), core-shell bait-containing nanoparticles can perform three functions in one step: (a) sieve molecules according to size, (b) sequestrate and concentrate target analytes, and (c) protect analytes from degradation.