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{"title":"Isolation of Mitochondria from the CNS","authors":"Tibor Kristian","doi":"10.1002/0471142301.ns0722s52","DOIUrl":null,"url":null,"abstract":"<p>This unit contains a protocol describing the isolation of brain mitochondria by using discontinuous Percoll gradient centrifugation. The Percoll density gradient centrifugation separates synaptosomes, myelin, and free nonsynaptic mitochondria released from cells during tissue homogenization into individual fractions. Mitochondria entrapped in synaptosomes (synaptic mitochondria) can be liberated using nitrogen cavitation and then further purified by Percoll gradient centrifugation. These methods yield mitochondria that exhibit good respiratory coupling and high respiratory rates. <i>Curr. Protoc. Neurosci</i>. 52:7.22.1-7.22.12. © 2010 by John Wiley & Sons, Inc.</p>","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/0471142301.ns0722s52","citationCount":"60","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Neuroscience","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/0471142301.ns0722s52","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Neuroscience","Score":null,"Total":0}
引用次数: 60
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Abstract
This unit contains a protocol describing the isolation of brain mitochondria by using discontinuous Percoll gradient centrifugation. The Percoll density gradient centrifugation separates synaptosomes, myelin, and free nonsynaptic mitochondria released from cells during tissue homogenization into individual fractions. Mitochondria entrapped in synaptosomes (synaptic mitochondria) can be liberated using nitrogen cavitation and then further purified by Percoll gradient centrifugation. These methods yield mitochondria that exhibit good respiratory coupling and high respiratory rates. Curr. Protoc. Neurosci . 52:7.22.1-7.22.12. © 2010 by John Wiley & Sons, Inc.
中枢神经系统线粒体的分离
本单元包含描述脑线粒体分离的协议，使用不连续Percoll梯度离心。Percoll密度梯度离心分离法分离突触体、髓磷脂和游离的非突触线粒体，这些线粒体是在组织匀浆过程中从细胞中释放出来的。捕获在突触体中的线粒体(突触线粒体)可以用氮空化释放，然后用Percoll梯度离心进一步纯化。这些方法产生的线粒体表现出良好的呼吸耦合和高呼吸速率。咕咕叫。Protoc。> 52:7.22.1-7.22.12。©2010 by John Wiley &儿子,Inc。
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