[Detection of bacterial meningitis by multiplex polymerase chain reaction].

中国疫苗和免疫 Pub Date : 2010-02-01
Hong-Yu Ren, Shu-Yi An, Ma-Chao Li
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引用次数: 0

Abstract

Objective: To develop a rapid method for detecting 8 pathogens which were highly related to bacterial meningitis by multiplex polymerase chain reaction.

Method: By optimizing the reaction condition and amplification program of single pair polymerase chain reaction, the multiplex pairs polymerase chain reactions (M-PCR) was developed to identify eight pathogens simultaneously including Neisseria Meningitis, Haemophilus Influenzae, Streptococcus Pneumoniae, Cryptococcus Neoformans, Staphylococcus Aureus, Listerisa Monocytogene, Streptococcus Suis and Mycobacterium Tuberculosis. Meanwhile, The sensitivity of M-PCR assay was also studied.

Results: M-PCR methods for detecting 8 pathogens which could cause bacterial meningitis have been established. M-PCR showed specific, sensitive and more rapid than conventional culturing method.

Conclusion: This multiplex polymerase chain reaction method can be used for diagnosis and scanning of suspicious bacterial meningitis cases in order to improve the diagnostic positive rate of bacterial meningitis cases.

多重聚合酶链反应检测细菌性脑膜炎
目的:建立多重聚合酶链反应快速检测8种与细菌性脑膜炎高度相关病原体的方法。方法:通过优化单对聚合酶链反应的反应条件和扩增程序,建立多重对聚合酶链反应(M-PCR),同时鉴定脑膜炎奈瑟菌、流感嗜血杆菌、肺炎链球菌、新型隐球菌、金黄色葡萄球菌、单核细胞增生李斯特菌、猪链球菌和结核分枝杆菌8种病原菌。同时对M-PCR法的敏感性进行了研究。结果:建立了8种细菌性脑膜炎病原菌的M-PCR检测方法。与传统培养方法相比,M-PCR具有特异性、敏感性和快速性。结论:该多重聚合酶链反应法可用于细菌性脑膜炎可疑病例的诊断和扫描,以提高细菌性脑膜炎的诊断阳性率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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