Christian Westendorf, Albert J Bae, Christoph Erlenkamper, Edouard Galland, Carl Franck, Eberhard Bodenschatz, Carsten Beta
{"title":"Live cell flattening - traditional and novel approaches.","authors":"Christian Westendorf, Albert J Bae, Christoph Erlenkamper, Edouard Galland, Carl Franck, Eberhard Bodenschatz, Carsten Beta","doi":"10.1186/1757-5036-3-9","DOIUrl":null,"url":null,"abstract":"<p><p> Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.PACS Codes: 87.64.-t, 47.61.-k, 87.80.Ek.</p>","PeriodicalId":88297,"journal":{"name":"PMC biophysics","volume":"3 1","pages":"9"},"PeriodicalIF":0.0000,"publicationDate":"2010-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873278/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PMC biophysics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/1757-5036-3-9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.PACS Codes: 87.64.-t, 47.61.-k, 87.80.Ek.
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