[Development of a liquid chromatography-tandem mass spectrometry method for the determination of fullerenes C60 and C70 in biological samples].

Q4 Medicine

Bulletin of National Institute of Health Sciences Pub Date : 2009-01-01

Reiji Kubota, Maiko Tahara, Kumiko Shimizu, Naoki Sugimoto, Akihiko Hirose, Tetsuji Nishimura

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引用次数: 0

Abstract

Wide application of fullerenes in various areas would increase the risk of occupational and environmental exposure to human. However, information about toxicity and biological behavior of fullerenes is not sufficient for the risk assessment at present. For the determination of fullerene C60 in biological samples, an analytical method using high performance liquid chromatography--tandem mass spectrometry (LC-MS/MS) and extraction procedure from tissues of experimental animals was established in this study. Using LC-MS/MS with an atmospheric pressure chemical ionization in negative mode, C60 were identified and quantified. After optimization of mobile phase and separation column, good separation of peak of fullerene and sensitivity were obtained in case of using toluene and acetonitrile as the mobile phases and Develosil RPFULLERENE as the separation column. For method validation, rat brain, kidney, liver, lung, spleen tissues and blood were used for recovery tests. Good results were obtained and the recovery percentages were found to be between 98.1% and 106.5%.

本刊更多论文

[液相色谱-串联质谱法测定生物样品中富勒烯C60和C70的研究]。

富勒烯在各个领域的广泛应用会增加人类职业和环境暴露的风险。然而，目前关于富勒烯的毒性和生物学行为的信息还不足以进行风险评估。为了测定生物样品中的富勒烯C60，本研究建立了高效液相色谱-串联质谱(LC-MS/MS)分析方法和提取实验动物组织的方法。采用负模式常压化学电离LC-MS/MS对C60进行了鉴定和定量。通过对流动相和分离柱的优化，以甲苯和乙腈为流动相，以developsil RPFULLERENE为分离柱，获得了较好的富勒烯峰分离效果和灵敏度。为验证方法，采用大鼠脑、肾、肝、肺、脾组织和血液进行恢复试验。回收率在98.1% ~ 106.5%之间，效果良好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of National Institute of Health Sciences Medicine-Medicine (all)

CiteScore

0.20

自引率

0.00%

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