Conjugating Methotrexate to magnetite (Fe(3)O(4)) nanoparticles via trichloro-s-triazine.

Kaylie L Young, Chenjie Xu, Jin Xie, Shouheng Sun
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引用次数: 47

Abstract

Monodisperse Fe(3)O(4) nanoparticles (NPs) originally synthesized with a hydrophobic oleylamine capping ligand were made water soluble and conjugated to the anticancer drug Methotrexate (MTX) using a new chemistry based on the readily available linker trichloro-s-triazine (TsT). This new linker is much more versatile than those that currently exist for attaching biomolecules to magnetic NPs. The MTX-conjugated NPs were found to be stable under physiological conditions for over 72 hours and MTX was shown to maintain its anticancer activity after conjugation to the NP surface. Through cell viability studies and intracellular uptake studies, MTX-conjugated NPs were shown to have targeting specificity for a tumor cell line (9L rat glioma) over a healthy cell line (Cultured Pulmonary Artery Endothelial). Additionally the MTX-conjugated NPs were visualized inside 9L cells using fluorescence microscopy to help elucidate their path within a cell after internalization.

三氯-s-三嗪偶联甲氨蝶呤与磁铁矿(Fe(3)O(4))纳米颗粒。
单分散的铁(3)O(4)纳米颗粒(NPs)最初是由疏水性的油胺盖层配体合成的,现在通过一种新的化学方法,以现成的连接剂三氯三嗪(TsT)为基础,使其可溶于水,并与抗癌药物甲氨蝶呤(MTX)结合。这种新的连接体比目前存在的将生物分子连接到磁性NPs上的连接体更加通用。MTX偶联的NP在生理条件下稳定72小时以上,MTX偶联到NP表面后仍能保持其抗癌活性。通过细胞活力研究和细胞内摄取研究,证明mtx结合的NPs对肿瘤细胞系(9L大鼠胶质瘤)比健康细胞系(培养肺动脉内皮细胞)具有靶向特异性。此外,利用荧光显微镜在9L细胞内观察mtx共轭的NPs,以帮助阐明其内化后在细胞内的路径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Materials Chemistry
Journal of Materials Chemistry 工程技术-材料科学:综合
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1.5 months
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