Expression of antiviral molecular genes in nasal polyp-derived cultured epithelial cells.

Acta oto-laryngologica. Supplementum Pub Date : 2009-06-01 DOI:10.1080/00016480902912001

So Watanabe, Jiyun Wang, Satoshi Matsukura, Harumi Suzaki

{"title":"Expression of antiviral molecular genes in nasal polyp-derived cultured epithelial cells.","authors":"So Watanabe, Jiyun Wang, Satoshi Matsukura, Harumi Suzaki","doi":"10.1080/00016480902912001","DOIUrl":null,"url":null,"abstract":"Conclusion: The results of the present study indicate the existence of natural immunity mechanisms via which viruses are eliminated from nasal and paranasal sinus mucosa.Objectives: Acute sinusitis and acute aggravation of chronic sinusitis are often caused by bacteria, which are secondary to viral infection of the nose. Antiviral molecules are considered to be expressed and protect the host after viral infection. We investigated the expression of antiviral molecules after viral infection of the nose.Materials and methods: We assessed the expression of antiviral molecules, defensin and interferon mRNA, by the real-time polymerase chain reaction (PCR) technique after stimulating cultured nasal polyp cells with polyinosine-polycytidylic acid (poly(I:C)), which is an analog of double-stranded (ds) RNA.Results: The expression of beta-defensin mRNA significantly increased after the stimulation. On the other hand, expression of both interferon-alpha mRNA and interferon-beta mRNA was recognized, but only the expression of interferon-beta mRNA increased after the stimulation.","PeriodicalId":7027,"journal":{"name":"Acta oto-laryngologica. Supplementum","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00016480902912001","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta oto-laryngologica. Supplementum","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/00016480902912001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 3

Abstract

Conclusion: The results of the present study indicate the existence of natural immunity mechanisms via which viruses are eliminated from nasal and paranasal sinus mucosa.

Objectives: Acute sinusitis and acute aggravation of chronic sinusitis are often caused by bacteria, which are secondary to viral infection of the nose. Antiviral molecules are considered to be expressed and protect the host after viral infection. We investigated the expression of antiviral molecules after viral infection of the nose.

Materials and methods: We assessed the expression of antiviral molecules, defensin and interferon mRNA, by the real-time polymerase chain reaction (PCR) technique after stimulating cultured nasal polyp cells with polyinosine-polycytidylic acid (poly(I:C)), which is an analog of double-stranded (ds) RNA.

Results: The expression of beta-defensin mRNA significantly increased after the stimulation. On the other hand, expression of both interferon-alpha mRNA and interferon-beta mRNA was recognized, but only the expression of interferon-beta mRNA increased after the stimulation.

查看原文本刊更多论文

鼻腔息肉源性上皮细胞中抗病毒分子基因的表达。

结论:本研究结果表明存在一种自然免疫机制，病毒可通过该机制从鼻和副鼻窦粘膜中清除。目的:急性鼻窦炎和慢性鼻窦炎急性加重常由细菌引起，继发于鼻腔病毒感染。抗病毒分子被认为在病毒感染后表达并保护宿主。我们研究了鼻腔病毒感染后抗病毒分子的表达。材料与方法:采用实时聚合酶链反应(real-time polymerase chain reaction, PCR)技术，用双链RNA (ds)类似物聚肌苷-多胞酸(poly(I:C))刺激培养鼻息肉细胞后，检测抗病毒分子、防御素和干扰素mRNA的表达。结果:刺激后β -防御素mRNA表达显著升高。另一方面，干扰素- α mRNA和干扰素- β mRNA的表达均被识别，但刺激后只有干扰素- β mRNA的表达增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta oto-laryngologica. Supplementum

自引率

0.00%

发文量