Photoelectrochemical identification of 5-methylcytosine modification in DNA: combination of photosensitization and enzymatic cleavage.

Kazuhito Tanabe, Hisatsugu Yamada, Takeo Ito, Sei-ichi Nishimoto
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Abstract

We present a novel photoelectrochemical approach for discriminating between cytosine and 5-methylcytosine in DNA when used in combination with enzymatic digestion. A photosensitizer-linked DNA duplex bearing 5-methylcytosine or cytosine at a given restriction site of the strand was immobilized on a gold electrode and digested with enzyme, and the photocurrent response was measured. We observed high photocurrent density that was selective for the methylated duplex.

DNA中5-甲基胞嘧啶修饰的光电化学鉴定:光敏和酶切的结合。
我们提出了一种新的光电化学方法来区分DNA中的胞嘧啶和5-甲基胞嘧啶,当与酶切结合使用时。将光敏剂连接的DNA双链固定在金电极上,用酶消化,测量光电流响应。我们观察到高光电流密度对甲基化双相具有选择性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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