The position of repetitive DNA sequence in the southern cattle tick genome permits chromosome identification.

Catherine A Hill, Felix D Guerrero, Janice P Van Zee, Nicholas S Geraci, Jason G Walling, Jeffrey J Stuart
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引用次数: 20

Abstract

Fluorescent in-situ hybridization (FISH) using meiotic chromosome preparations and highly repetitive DNA from the southern cattle tick, Rhipicephalus microplus, was undertaken to investigate genome organization. Several classes of highly repetitive DNA elements were identified by screening a R. microplus bacterial artificial chromosome (BAC) library. A repeat unit of approximately 149 bp, RMR-1 was localized to the subtelomeric regions of R. microplus autosomes 1-6 and 8-10. A second repeat unit, RMR-2 was localized to the subtelomeric regions of all autosomes and the X chromosome. RMR-2 was composed of three distinct repeat populations, RMR-2a, RMR-2b and RMR-2c of 178, 177 and 216 bp in length, respectively. Localization of an rDNA probe identified a single nucleolar organizing region on one autosome. Using a combination of labeled probes, we developed a preliminary karyotype for R. microplus. We present evidence that R. microplus has holocentric chromosomes and explore the implications of these findings for tick chromosome biology and genomic research.

南方牛蜱基因组中重复DNA序列的位置允许染色体鉴定。
利用南方牛蜱(Rhipicephalus microplus)的减数分裂染色体和高度重复DNA,进行了荧光原位杂交(FISH)研究。通过筛选microplus细菌人工染色体文库,鉴定出了几种高度重复的DNA元件。RMR-1的重复单位约为149 bp,定位于微鼠常染色体1-6和8-10的亚端粒区。第二个重复单元,RMR-2定位于所有常染色体和X染色体的亚端粒区域。RMR-2由长度分别为178、177和216 bp的RMR-2a、RMR-2b和RMR-2c三个不同的重复种群组成。rDNA探针的定位鉴定了一个常染色体上的单个核仁组织区。利用标记探针的组合,我们建立了一个初步的染色体核型。我们提出证据表明微蜱具有全新中心染色体,并探讨这些发现对蜱染色体生物学和基因组研究的意义。
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