S Kitahashi, N Tatsumi, S Tagawa, T Matsui, M Higashihata, H Shintaku, S Tomoda, I Tsuda
{"title":"Diagnosis of infections in newborns using a new particle-mediated immunoassay for serum C-reactive protein.","authors":"S Kitahashi, N Tatsumi, S Tagawa, T Matsui, M Higashihata, H Shintaku, S Tomoda, I Tsuda","doi":"10.1155/S146392469800025X","DOIUrl":null,"url":null,"abstract":"<p><p>C-reactive protein (CRP) levels were measured using a new particle-mediated immunoassay. Tests for precision and linearity of this method gave satisfactory results. The minimum sensitivity of the assay was 1 ng/ml. Interference by bilirubin (<220mg/l) and haemoglobin (<20g/l) was not observed. Using this method, CRP was assayed as a means of monitoring for infection in newborns up to 72 h after delivery. The pattern of time course elevation curves was similar for both groups (10 healthy subjects and 26 patients), but the serum CRP (ng/ml) of infected newborns rose significantly higher than in healthy subjects at 24 h after birth. The rate of increase of CRP (CRP; ng/ml/h) may be a more useful parameter to detect infection, since a significant change in CRP was apparent only 12 h after birth. The reported method was reliable and the parameters obtained were considered clinically useful for early detection of infection.</p>","PeriodicalId":22600,"journal":{"name":"The Journal of Automatic Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/S146392469800025X","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Automatic Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/S146392469800025X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
C-reactive protein (CRP) levels were measured using a new particle-mediated immunoassay. Tests for precision and linearity of this method gave satisfactory results. The minimum sensitivity of the assay was 1 ng/ml. Interference by bilirubin (<220mg/l) and haemoglobin (<20g/l) was not observed. Using this method, CRP was assayed as a means of monitoring for infection in newborns up to 72 h after delivery. The pattern of time course elevation curves was similar for both groups (10 healthy subjects and 26 patients), but the serum CRP (ng/ml) of infected newborns rose significantly higher than in healthy subjects at 24 h after birth. The rate of increase of CRP (CRP; ng/ml/h) may be a more useful parameter to detect infection, since a significant change in CRP was apparent only 12 h after birth. The reported method was reliable and the parameters obtained were considered clinically useful for early detection of infection.