Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering

Biomolecular engineering Pub Date : 2007-12-01 DOI:10.1016/j.bioeng.2007.08.019

Michela Bosetti , Francesca Boccafoschi , Massimiliano Leigheb , Mario F. Cannas

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引用次数: 66

Abstract

Cultured human primary osteoblasts reproduce the phenotypic differentiation and maturation of cells in vivo. We have investigated the influence of three isoforms of transforming growth factor beta (TGF-β1, TGF-β2 and TGF-β3), three fibroblast growth factors (FGF-2, FGF-4 and FGF-6) and the active metabolite of Vitamin D [1,25-(OH)₂D3] on proliferation, alkaline phosphatase activity and mineralization of human osteoblasts during a period of 24 days of culture. TGF-β isoforms and three FGFs examined have been proved to be inducers of osteoblasts proliferation (higher extent for TGF-β and FGF-2) and inhibitors of alkaline phosphatase activity and osteoblasts mineralization. Combination of these growth factors with the active form of Vitamin D induced osteodifferentiation. In fact Vitamin D showed an additive effect on alkaline phosphatase activity and calcium content, induced by FGF-2 and TGF-β in human osteoblast. These results highlight the potential of proliferating cytokines’ combination with mineralizing agents for in vitro bone growth induction in bone tissue engineering.

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不同生长因子对人成骨细胞活性的影响:在组织工程骨再生中的可能应用

培养的人原代成骨细胞在体内复制细胞的表型分化和成熟。我们研究了转化生长因子β三种亚型(TGF-β1、TGF-β2和TGF-β3)、成纤维细胞生长因子三种亚型(FGF-2、FGF-4和FGF-6)和维生素D活性代谢物[1,25-(OH)2D3]在24天培养过程中对人成骨细胞增殖、碱性磷酸酶活性和矿化的影响。TGF-β亚型和三种FGFs已被证明是成骨细胞增殖的诱导剂(TGF-β和FGF-2的程度更高)和碱性磷酸酶活性和成骨细胞矿化的抑制剂。这些生长因子与活性形式的维生素D结合诱导骨分化。事实上，维生素D对人成骨细胞中FGF-2和TGF-β诱导的碱性磷酸酶活性和钙含量具有加性作用。这些结果突出了增殖细胞因子联合矿化剂在骨组织工程中体外诱导骨生长的潜力。

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Biomolecular engineering

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