Evaluation of a collagen-glycosaminoglycan dermal substitute in the dog palate.

Ricardo Ophof, Jaap C Maltha, Anne-Marie Kuijpers-Jagtman, Johannes W Von Den Hoff
{"title":"Evaluation of a collagen-glycosaminoglycan dermal substitute in the dog palate.","authors":"Ricardo Ophof,&nbsp;Jaap C Maltha,&nbsp;Anne-Marie Kuijpers-Jagtman,&nbsp;Johannes W Von Den Hoff","doi":"10.1089/ten.2006.0368","DOIUrl":null,"url":null,"abstract":"<p><p>Tissue shortage complicates surgery of cleft lip and palate. The healing of defects on the palate impairs growth of the dentoalveolar complex because of scar tissue formation. Implantation of a matrix into the wound might overcome this adverse effect. Integra with and without a silicone top layer was implanted into standardized full-thickness wounds (Ø 6 mm) in the palatal mucoperiosteum in beagle dogs. In some wounds, the silicone layer was removed after 14 days. Control wounds did not have an implant. At 2 and 4 weeks post-surgery, the wounds were assessed for epithelialization, inflammation (hematoxylin and eosin, leucocyte protein L1), number of myofibroblasts (alpha smooth muscle actin), and general histological characteristics. Wounds filled with Integra without the silicone layer showed fewer myofibroblasts and inflammatory cells than the sham wounds. Collagen fibers were more randomly orientated in these wounds than in the sham group. Wound closure was found to be retarded, and many inflammatory cells were present when Integra with silicone was implanted. The silicone layer was lost within 4 weeks in these wounds. We conclude that, in the moist oral environment, the silicone of Integra is not required. Re-epithelialization and tissue integration proceed more favorably without it. Further research in the dentoalveolar development with Integra will be conducted in a simulated cleft palate repair in the dog model.</p>","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":"13 11","pages":"2689-98"},"PeriodicalIF":0.0000,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2006.0368","citationCount":"17","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/ten.2006.0368","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17

Abstract

Tissue shortage complicates surgery of cleft lip and palate. The healing of defects on the palate impairs growth of the dentoalveolar complex because of scar tissue formation. Implantation of a matrix into the wound might overcome this adverse effect. Integra with and without a silicone top layer was implanted into standardized full-thickness wounds (Ø 6 mm) in the palatal mucoperiosteum in beagle dogs. In some wounds, the silicone layer was removed after 14 days. Control wounds did not have an implant. At 2 and 4 weeks post-surgery, the wounds were assessed for epithelialization, inflammation (hematoxylin and eosin, leucocyte protein L1), number of myofibroblasts (alpha smooth muscle actin), and general histological characteristics. Wounds filled with Integra without the silicone layer showed fewer myofibroblasts and inflammatory cells than the sham wounds. Collagen fibers were more randomly orientated in these wounds than in the sham group. Wound closure was found to be retarded, and many inflammatory cells were present when Integra with silicone was implanted. The silicone layer was lost within 4 weeks in these wounds. We conclude that, in the moist oral environment, the silicone of Integra is not required. Re-epithelialization and tissue integration proceed more favorably without it. Further research in the dentoalveolar development with Integra will be conducted in a simulated cleft palate repair in the dog model.

胶原-糖胺聚糖真皮替代物在狗腭中的应用。
组织缺乏是唇腭裂手术的难点。由于瘢痕组织的形成,腭裂的愈合损害了牙槽嵴复合体的生长。将基质植入伤口可能会克服这种不利影响。在beagle犬腭粘骨膜标准化全层创面(Ø 6 mm)中植入有或没有硅胶顶层的Integra。在一些伤口中,硅胶层在14天后被移除。对照伤口没有植入物。术后2周和4周,评估伤口上皮化、炎症(苏木精和伊红、白细胞蛋白L1)、肌成纤维细胞(α平滑肌肌动蛋白)数量和一般组织学特征。没有硅酮层的Integra填充伤口的肌成纤维细胞和炎症细胞比假伤口少。与假手术组相比,胶原纤维在这些伤口中的定向更加随机。植入Integra硅胶后发现伤口愈合迟缓,并出现许多炎症细胞。这些伤口的硅胶层在4周内就消失了。我们的结论是,在潮湿的口腔环境中,Integra的硅胶是不需要的。没有它,再上皮化和组织整合进行得更顺利。我们将在模拟狗的腭裂修复模型中对Integra牙槽发育进行进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信