[Study on the expression of matrix metalloproteinase-12 and NM_002426 in squamous laryngeal carcinoma].

Abstract

Objective: Matrix metalloproteinases (MMPs) are key factors which influence the grades and/or metastasis of stereo tumour. There are short of research of MMP-12 now. The experiment is done to investigate the expression of MMP-12 and NM_002426, and relationship between the two genes and the biologic behavior in squamous laryngeal carcinoma.

Method: Thirty pairs of specimens, include supraglottic carcinoma tissue and normal mucosa, were chosen. Three gene arrays, based on different kinds of malignant, were made to study NM_ 002426 at first, then immunohistochemical method were used to observe the difference of MMP-12 between tumour and normal mucosa, metastasis group and no-metastasis group.

Result: NM_002426 express high ratio in all 3 chips, and have the highest mark in both mid-level and high-level malignant tissue. The immunohistochemical results showed that MMP-12 had significant difference between tumour and normal mucosa, metastasis group and no-metastasis group. The MMP-12 expression was unrelated to different malignant degree.

Conclusion: NM_ 002426 has significant hyperplasia in squamous laryngeal carcinoma, which caused high expression of MMP-12,and it makes a main contribution to the carcinogenesis and metastasis of laryngeal carcinoma. MMP-12 and its gene overexpress may be a auxiliary diagnosis method.