[The role of serine endopeptidase in cucumber leaf senescence].

植物生理与分子生物学学报 Pub Date : 2006-10-01
Peng Zhang, Fei Wang, Lie-Feng Zhang, Qi Rui, Lang-Lai Xu
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Abstract

The role of serine endopeptidase in cucumber leaf senescence was studied by using the inhibitor of serine endopeptidase and plant growth regulators (6-BA and ABA) on darkness-induced cucumber leaves. The results showed that the senescence of cucumber leaves were delayed by AEBSF [4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride], an inhibitor of serine-type endopeptidase, or 6-BA treatment. The chlorophyll contents increased by AEBSF (Fig.3) and the protein degradation of leaves under AEBSF treatment declined more slowly than in the control or under ABA 50 micromol/L treatment (Fig.4), partly because the activities of serine endopeptidases became lower during senescence. However, the activities of endopeptidase in cucumber leaf were increased by ABA 50 micromol/L (Fig.2A), furthermore, the MDA content were also influenced by AEBSF and plant growth regulators (Fig.5). Native gradient PAGE showed that six bands of isoenzymes were detected in cucumber leaves and four bands of which were the type of serine-endopeptidase (Fig.1), and proved that the activities of serine-endopeptidase were inhibited by AEBSF, but enhanced by ABA (Fig.2B) in the leaves. It implies that serine endopeptidases might play an important role in cucumber leaf senescence.

[丝氨酸内肽酶在黄瓜叶片衰老中的作用]。
通过使用丝氨酸内肽酶抑制剂和植物生长调节剂(6-BA 和 ABA)对暗诱导的黄瓜叶片进行研究,探讨丝氨酸内肽酶在黄瓜叶片衰老中的作用。结果表明,丝氨酸型内肽酶抑制剂 AEBSF[4-(2-氨基乙基)苯磺酰氟盐酸盐]或 6-BA 可延缓黄瓜叶片的衰老。在 AEBSF 的作用下,叶绿素含量增加(图 3),与对照或 ABA 50 微摩尔/升处理相比,AEBSF 处理下叶片蛋白质降解的速度更慢(图 4),部分原因是丝氨酸内肽酶的活性在衰老过程中变得更低。然而,ABA 50 微摩尔/升会提高黄瓜叶片内肽酶的活性(图 2A),而且 MDA 含量也受 AEBSF 和植物生长调节剂的影响(图 5)。原生梯度 PAGE 显示,黄瓜叶片中检测到 6 条同工酶条带,其中 4 条为丝氨酸内肽酶类型(图 1),证明丝氨酸内肽酶的活性受 AEBSF 抑制,但受 ABA 影响而增强(图 2B)。这意味着丝氨酸内肽酶可能在黄瓜叶片衰老过程中发挥了重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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