Standardization and quality control for the determination of alpha-glucosidase in seminal plasma.

Archives of andrology Pub Date : 2006-11-01 DOI:10.1080/01485010600822705

J-C Lu, H-R Xu, F Chen, Y-F Huang, N-Q Lu

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引用次数: 7

Abstract

We attempted to provide an instruction for the standardization of alpha-glucosidase level determination and quality controls within the same laboratory and among different laboratories. Each of 51 semen samples was divided into two aliquots, centrifuging at 1000 g for 10 min or 3000 g for 15 min. The alpha-glucosidase level and sperm concentration of each sample were assayed. The alpha-glucosidase level in seminal plasma obtained at 3000 g for 15 min centrifugation was significantly lower than that at 1000 g for 10 min (p = 0.001). An additional 6 samples of seminal plasma with or without phenylmethylsulfonyl fluoride (PMSF), obtained at 3000 g for 15 min centrifugation, were frozen for 20 days, and each of 6 samples was determined for their alpha-glucosidase levels after thawing every other day. There was no significant difference between alpha-glucosidase levels in seminal plasma regardless of the presence of PMSF. The alpha-glucosidase level increased with the length of abstinence period. In conclusion, centrifugal velocity and abstinence time should be standardized in the alpha-glucosidase determination. Frozen seminal plasma may serve as the sample for quality control among clinical laboratories.

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精浆中α -葡萄糖苷酶测定的标准化和质量控制。

我们试图为同一实验室和不同实验室间α -葡萄糖苷酶水平测定和质量控制的标准化提供指导。将51份精液样本分成两份，1000 g离心10 min或3000 g离心15 min，检测每份样本的α -葡萄糖苷酶水平和精子浓度。3000 g离心15 min时精浆α -葡萄糖苷酶水平显著低于1000 g离心10 min时(p = 0.001)。另外6份含或不含苯基甲基磺酰氟(PMSF)的精浆样品，在3000 g离心15分钟后冷冻20天，每隔一天解冻后检测6份样品的α -葡萄糖苷酶水平。无论PMSF是否存在，精浆中α -葡萄糖苷酶水平均无显著差异。α -葡萄糖苷酶水平随戒断时间的延长而升高。综上所述，在α -葡萄糖苷酶测定中，离心速度和停药时间应标准化。冷冻精浆可作为临床实验室质量控制的样本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Archives of andrology

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