[Expression analysis of different collagens and cytokines in cartilage cells derived from arthrotic hip and knee joints].

M Hoberg, W K Aicher, N Wülker, M Rudert
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引用次数: 2

Abstract

Aim: Osteoarthritis (OA) is characterized by an irreversible destruction of articular cartilage. This is associated with a multiplicity of factors, causing an increased catabolic metabolism in cartilage. However, the prevalence of the OA is very variable in different joints. Therefore , we conducted a comparative analysis of chondrocytes derived from knee and hip joints with respect to their expression of inflammatory factors, such as IL-1beta, IL-1beta-receptorantagonist, iNOS, components of cartilage matrix (collagen I, II, and VI) as well as vimentin.

Methods: Different cytokines and proteins were detected by immune-histochemical staining of cartilage samples ex vivo. Further, chondrocytes were isolated from OA knee and hip joints, expanded in vitro and gene expression patterns were investigated by quantitative RT-PCR.

Results: Chondrocytes from knee and hip joints of OA patients express collagenes I, II and VI, IL-1beta and IL-1beta-RA, iNOS as well as Vimentin. A significant difference in gene expression patterns was not found in chondrocytes from the hip joints versus the knee joint ex vivo or in primary culture cells in vitro. However, in vitro the expression of type I collagen exceeded the expression of type II collagen. The IL-1beta-expression was high ex vivo, remained low during primary culture but was significantly elevated after primary culture in hip chondrocytes.

Conclusion: Osteoarthritic gene expression patterns in cells derived from hip or knee joints ex vivo and in primary culture were not significantly different. We conclude that the rather frequent occurrence of OA in these joints in comparison to the ankle joint may be associated with a close physiological relation of cells in these joints. However, future studies which will include ankle cartilage must be investigated in further detail.

[髋关节和膝关节软骨细胞中不同胶原和细胞因子的表达分析]。
目的:骨关节炎(OA)的特点是关节软骨的不可逆破坏。这与多种因素有关,导致软骨分解代谢增加。然而,骨性关节炎的患病率在不同的关节是非常不同的。因此,我们对膝关节和髋关节软骨细胞的炎症因子表达进行了比较分析,如il -1 β、il -1 β受体拮抗剂、iNOS、软骨基质成分(胶原I、II和VI)以及波形蛋白。方法:采用体外免疫组化染色法检测软骨组织中不同细胞因子和蛋白的含量。此外,从OA膝关节和髋关节中分离软骨细胞,体外扩增,并通过定量RT-PCR研究基因表达模式。结果:OA患者膝关节、髋关节软骨细胞表达胶原I、II、VI、il -1 β、il -1 β - ra、iNOS、Vimentin。在髋关节软骨细胞和膝关节软骨细胞的离体或原代培养细胞中,基因表达模式没有发现显著差异。然而,在体外,I型胶原的表达超过II型胶原的表达。il -1 β在体外高表达,在原代培养期间保持低表达,但在髋关节软骨细胞原代培养后显著升高。结论:骨关节炎基因在髋关节或膝关节细胞的离体表达与原代培养无显著差异。我们得出结论,与踝关节相比,这些关节中OA的发生率较高,可能与这些关节中细胞的密切生理关系有关。然而,未来的研究将包括踝关节软骨必须进行更详细的调查。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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