Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Discovery in Aging and Alzheimer’s Disease

NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics Pub Date : 2006-07-01 DOI:10.1016/j.nurx.2006.05.002

Thomas J. Montine, Randall L. Woltjer, Catherine Pan, Kathleen S. Montine, Jing Zhang

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引用次数: 14

Abstract

Summary

Systems biology offers enormous potential to understand the complexity of human brain aging and neurodegenerative diseases. Proteomics has an important role in these investigations because of its unique strengths and because of the potential central pathogenic contribution of pathological protein to several of these diseases. Here we have reviewed the methods and presented some examples of liquid chromatography–electrospray ionization–tandem mass spectrometry–based proteomics, with and without quantification using isotope-coded affinity tags, in the investigation of aging and Alzheimer’s disease. As protocols and methods for improved quantitative high-throughput proteomics constantly improve, this approach will likely continue to provide deeper insight into human brain aging and neurodegenerative diseases.

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基于液相色谱和串联质谱的蛋白质组学在衰老和阿尔茨海默病中的发现

系统生物学为理解人类大脑老化和神经退行性疾病的复杂性提供了巨大的潜力。蛋白质组学在这些研究中发挥着重要作用，因为它具有独特的优势，也因为病理蛋白对这些疾病的潜在核心致病作用。在此，我们回顾了基于液相色谱-电喷雾电离-串联质谱的蛋白质组学方法，并介绍了一些使用同位素编码亲和标签进行定量和不进行定量的例子，以研究衰老和阿尔茨海默病。随着改进的定量高通量蛋白质组学的方案和方法的不断完善，这种方法可能会继续为人类大脑衰老和神经退行性疾病提供更深入的了解。

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NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics

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