Glial cytoarchitecture in the central nervous system of the soft-shell turtle, Trionyx sinensis, revealed by intermediate filament immunohistochemistry.

Abstract

The distribution of the intermediate filament molecular markers, glial fibrillary acidic protein (GFAP) and vimentin, has been studied in the central nervous system (CNS) of the soft-shell turtle (Trionyx sinensis) with immunoperoxidase histochemistry. GFAP immunohistochemistry pointed out the presence of different astroglial cell types. The brain pattern consists of ependymal radial glia whose cell bodies are located in the ependymal layer throughout the brain ventricular system. In the spinal cord, the ependyma is immunonegative, whereas positive radial astrocyte cell bodies are displaced from the ependyma into the periependymal position. Star-shaped astrocytes are observed only in the posterior intumescence of the spinal cord. The different regions of the CNS show a different intensity in GFAP immunostaining even in the same cellular type. Vimentin-immunoreactive structures are absent in the brain and spinal cord. The present study reports an heterogeneous feature of the astroglial pattern in the spinal cord compared to the brain which shows an ancestral condition.