The effect of RAB5A gene on rearrangement of microfilaments in human lung adenocarcinoma cells.

Zhong-Cheng Shi, Yang Yu, Yu Li, Song-Bin Fu
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Abstract

To study the effect of RAB5A gene on microfilaments in human lung adenocarcinoma cells, AGZY83-a cells were stained with fluorescein isothiocyanate (FITC)-phalloidin. Microfilament bundles in RAB5A gene over-expressed AGZY83-a cells were shown to be denser than in control cells using confocal laser scanning microscope (CLSM). According to the analysis of Tumor Metastasis Microarray on RAB5A gene, three genes related to the regulation of cytoskeleton were identified, including NM23H1, Rac1 (downregulated in RAB5A over expressing cells), and S100A4 (upregulated in RAB5A overexpressing cells). Previous studies demonstrated that S100A4 gene functioned to suppress the expression of NM23H1 gene. To test if this elevated expression of S100A4 results in the down-regulation of NM23H1, RNAi was applied to silence the expression of S100A4 in AGZY83-a cells. Our data indicated that expression of NM23H1 was increased following the inhibition of S100A4 expression. Altogether, the results indicated that RAB5A gene was involved in the suppression of expression of NM23H1 by promoting the expression of S100A4.

RAB5A基因对人肺腺癌细胞微丝重排的影响。
为了研究RAB5A基因对人肺腺癌细胞微丝的影响,采用异硫氰酸荧光素(FITC)-phalloidin对AGZY83-a细胞进行染色。共聚焦激光扫描显微镜(CLSM)显示RAB5A基因过表达AGZY83-a细胞的微丝束密度高于对照细胞。通过肿瘤转移微阵列对RAB5A基因的分析,鉴定出3个与细胞骨架调控相关的基因,分别是NM23H1、Rac1 (RAB5A过表达细胞下调)和S100A4 (RAB5A过表达细胞上调)。既往研究表明,S100A4基因具有抑制NM23H1基因表达的功能。为了验证S100A4的表达升高是否会导致NM23H1的下调,我们在AGZY83-a细胞中应用RNAi沉默S100A4的表达。我们的数据表明,抑制S100A4的表达后,NM23H1的表达增加。综上所述,RAB5A基因通过促进S100A4的表达参与抑制NM23H1的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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