[Development of transgenic oilseed plants resistant to glyphosate and insects].

Jing-Xue Wang, Fu-Yong Zhao, Pei-Lin Xu, Ying-Chuan Tian
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Abstract

In this study, the aroA-M12 gene encoding bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and the Btslm recombinant gene encoding Bacillus thuringiensis (Bt) toxin gene were introduced into a Brassica napus variety, Xiangyou No. 15, via Agrobacterium-mediated transformation using glyphosate as a selectable agent. PCR amplification and Southern blot analyses of T0 transgenic plants showed that the alien genes were transferred and integrated stably into the genome of Xiangyou No. 15. Western blot further confirmed that the alien genes were expressed in these plants. Transgenic plants with tolerance to both glyphosate and the tested pest were demonstrated by bioassays. Our result also clearly shows that the aroA-M12 gene can be used as an efficient selectable marker gene instead of antibiotic resistant markers in plant transformation.

[开发抗草甘膦和虫害的转基因油菜籽植物]。
本研究以草甘膦为选择剂,通过农杆菌介导转化法将编码细菌5-烯醇丙酮酰莽草酸-3-磷酸合成酶(EPSPS)的roA-M12基因和编码苏云金芽孢杆菌(Bt)毒素基因的Btslm重组基因导入甘蓝型油菜品种 "湘油15号"。对 T0 转基因植株进行的 PCR 扩增和 Southern 印迹分析表明,外来基因已转移并稳定整合到 "湘油 15 号 "的基因组中。Western 印迹进一步证实,外来基因在这些植株中得到了表达。生物测定证明,转基因植株对草甘膦和受试害虫都具有耐受性。我们的研究结果还清楚地表明,在植物转化过程中,roA-M12 基因可作为一种高效的选择性标记基因,取代抗生素标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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