Molecular marker assisted selection and pyramiding of two QTLs for fiber strength in upland cotton.

Wang-Zhen Guo, Tian-Zhen Zhang, Ye-Zhang Ding, Yi-Chao Zhu, Xin-Lian Shen, Xie-Fei Zhu
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Abstract

Based on two major QTLs that control high fiber strength which originated from an elite fiber germ-plasm line 7235 (Gossypium hiusutum L.), the efficiency of molecular marker-assisted selection (MAS) was investigated using two populations from pedigree selection and modified backcrossing pyramiding developed for the breeding purpose. Simian 3 (SM3), a widely planted variety in the Yangtze River Valley, and 7235 were used as parents to develop the two populations. In the two major QTLs for fiber strength from 7235, QTLfs-1 could explain more than 30% of the phenotypic variation (PV) in the (7235 x TM-1) F2 population. QTLfs-2 was at first identified in another super quality fiber line HS427-10 from (HS427-10 x TM-1) F2 population with 12.5% of PV explanation,which was further also identified in 7235 line but was non-allelic with QTLfs-1. The result of molecular marker-assisted selection for fiber strength showed that the genetic effect of the QTLfs-1 was stable under different environmental conditions, and its molecular marker-assisted selection showed significant selective efficiency among breeding populations with different genetic backgrounds. QTLfs-2 also showed high selective efficiency in advanced generation populations though its effect was a little lower than the former. When QTLfs-1 was selected simultaneously with 2 molecular markers with known genetic distance, the selection efficiency for the fiber strength was greatly increased. The pyramiding for two QTLs that control high fiber strength by MAS greatly improved the selection efficiency for cotton fiber strength. This report provides a successful example of MAS pyramiding for QTL for favorable traits in breeding programs.

陆地棉纤维强度两个qtl的分子标记辅助选择与金字塔化。
以优质纤维种质系7235 (Gossypium hiusutum L.)的两个控制高纤维强度的主要qtl为基础,利用家系选择和改良回交金字塔两个群体对分子标记辅助选择(MAS)的效率进行了研究。以长江流域广泛种植的水稻品种思绵3号(SM3)和7235为亲本进行两个种群的发育。在7235的两个主要纤维强度qtl中,QTLfs-1可以解释(7235 × TM-1) F2群体中30%以上的表型变异(PV)。QTLfs-2首先在(HS427-10 × TM-1) F2群体的另一优质纤维系HS427-10中被鉴定出,PV解释率为12.5%,在7235系中也被鉴定出,但与QTLfs-1非等位基因。分子标记辅助选择结果表明,QTLfs-1在不同环境条件下的遗传效应是稳定的,其分子标记辅助选择在不同遗传背景的育种群体中具有显著的选择效率。QTLfs-2在高代群体中也表现出较高的选择效率,但其效应略低于前者。当QTLfs-1与2个已知遗传距离的分子标记同时选择时,纤维强度的选择效率大大提高。利用MAS对两个控制高纤维强度的qtl进行筛选,大大提高了棉纤维强度的选择效率。本报告提供了一个在育种计划中对有利性状的QTL进行MAS金字塔化的成功例子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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