Function analysis of promoter trapping system after inserted into cotton (Gossypium hirsutum L. ) genome.

Shuang-Xia Jin, Xian-Long Zhang, Yi-Chun Nie, Xiao-Ping Guo, Yu-Qiang Sun, Chao Huang, Shao-Guang Liang
{"title":"Function analysis of promoter trapping system after inserted into cotton (Gossypium hirsutum L. ) genome.","authors":"Shuang-Xia Jin,&nbsp;Xian-Long Zhang,&nbsp;Yi-Chun Nie,&nbsp;Xiao-Ping Guo,&nbsp;Yu-Qiang Sun,&nbsp;Chao Huang,&nbsp;Shao-Guang Liang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The technique of promoter trapping was developed to investigate its viability in cotton ( Gossypium hirsutum L.) functional genomics. 141 independent transformants of cotton were generated via Agrobacterium tumefaciens mediated transformation, of which 97% showed positive by PCR detection. The reporter, GUS gene, was expressed to different extent in different organs, with a frequency of 48% in roots, 9.2% in vascular bundles of stem, 5.2% in leaves, and 51% in flowers. Meanwhile, we discovered that there existed great differences in expression patterns among different transgenic lines. Their GUS expression patterns were organ- or tissue-specific or ubiquitous in all of the plants. The promoter trapping system developed here was characterized as an effective method for creating mutants with diverse reporter gene expression patterns, which laid a solid foundation for further research of functional genomics in cotton.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 12","pages":"1266-74"},"PeriodicalIF":0.0000,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Yi chuan xue bao = Acta genetica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The technique of promoter trapping was developed to investigate its viability in cotton ( Gossypium hirsutum L.) functional genomics. 141 independent transformants of cotton were generated via Agrobacterium tumefaciens mediated transformation, of which 97% showed positive by PCR detection. The reporter, GUS gene, was expressed to different extent in different organs, with a frequency of 48% in roots, 9.2% in vascular bundles of stem, 5.2% in leaves, and 51% in flowers. Meanwhile, we discovered that there existed great differences in expression patterns among different transgenic lines. Their GUS expression patterns were organ- or tissue-specific or ubiquitous in all of the plants. The promoter trapping system developed here was characterized as an effective method for creating mutants with diverse reporter gene expression patterns, which laid a solid foundation for further research of functional genomics in cotton.

棉种插入启动子诱捕系统的功能分析基因组。
为研究启动子诱捕技术在棉花(Gossypium hirsutum L.)中的活性,建立了启动子诱捕技术。通过农杆菌介导的棉花转化获得141个独立转化子,其中97%的转化子PCR检测阳性。报告基因GUS基因在不同器官中的表达频率不同,在根中表达频率为48%,在茎维管束中表达频率为9.2%,在叶中表达频率为5.2%,在花中表达频率为51%。同时,我们发现不同转基因系之间的表达模式存在很大差异。它们的GUS表达模式是器官或组织特异性的,或在所有植物中普遍存在。本研究建立的启动子诱捕系统是构建具有多种报告基因表达模式的突变体的有效方法,为棉花功能基因组学的进一步研究奠定了坚实的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信