Nuclear matrices and matrix attachment regions from Green alga: Dunaliella salina.

Tian-Yun Wang, Wei-Hong Hou, Yu-Rong Chai, Xiang Ji, Jian-Min Wang, Le-Xun Xue
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Abstract

Nuclear DNA of eukaryotic organism attaches to the proteinaceous nuclear matrices via specific matrix attachment regions (MARs). In order to investigate the interactions between chromosomal DNA and nuclear matrices,we isolated the MARs from unicellular alga Dunaliella salina. As the first step,a random MAR library was set up and then the binding affinity of the selected clones to nuclear matrices was tested in this study. Three DNA fragments were found to bind specifically to the nuclear matrices in vitro,of which two were strong binders and all contained known consensus motifs and a hairpin loop structure of MAR.

绿藻的核基质和基质附着区:盐藻杜氏藻。
真核生物的核DNA通过特定的基质附着区(MARs)附着在蛋白核基质上。为了研究染色体DNA与核基质之间的相互作用,我们从单细胞海藻Dunaliella salina中分离了MARs。本研究首先随机建立一个MAR文库,然后测试所选克隆与核基质的结合亲和力。在体外实验中发现三个DNA片段与核基质特异性结合,其中两个是强结合体,并且都含有已知的共识基序和MAR的发夹环结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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