Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene.

Abstract

Introduction: The expansion of specific trinucleotide repeats results in certain genetic disorders.

Method: Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene.

Result: The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C.

Conclusion: Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.