Intraperitoneal administration of an iodine-based contrast agent to improve abdominal micro-computed tomography imaging in mice.

Abstract

The purpose of this study was to estimate the optimal volume of an iodine-based contrast agent to administer to mice via intraperitoneal injection and the optimal time after injection to perform micro-computed tomography for maximal enhancement of abdominal organs. Eight mice were paired randomly; three pairs underwent imaging after receiving intraperitoneal injections of 125, 250, or 500 microl of contrast agent, and the fourth pair underwent imaging without receiving an injection. Each mouse was scanned three consecutive times, and each scan lasted 25 min so that we could observe the clearance of the contrast agent from the abdomen. We determined that introducing 250 microl of contrast agent into the abdominal cavity of the mice and then having the mice undergo micro-computed tomography 15 min after injection provided the optimal degree of contrast enhancement needed to distinguish the abdominal organs. These results may lead to expanded use of this imaging modality to assess abdominal organ margins in small-animal studies in vivo.