[Identification and molecular cytology analysis of novel wheat germplasm expressing seven high molecular weight glutenin subunits].

Abstract

This paper describes the characterization of novel wheat lines NR98116-9-2 and NR98116-9-3 derived from crosses of wheat with hexaploid triticale and expressing seven different high molecular weight glutenin subunits by SDS-PAGE analysis. The results suggested that the two lines were similar to wheat in morphological characters and were stable genetically. The chromosome number of root tip cells of the two lines were 2n=42 and 2n=44 and the configuration of the pollen mother cells at metaphase I were 21 // and 22 //, respectively. The analysis of chromosome constitution by in situ hybridization and C-banding led to the conclusion that NR98116-9-2 was a 3R (3D) disomic substitution line and NR98116-9-3 was a 3R disomic addition line. The HMW-GS compositions were 1, 14 + 15, 6r + 8, 4 + 12 and 1, 14 + 15, 6r + 8, 5 + 10, respectively. It is very interesting that both lines may contain two Glu-B1 sites. The potential usefulness of the two germplasm that expressed seven different high molecular weight glutenin subunits in wheat quality improvement was discussed.