{"title":"Cloning and characterization of a dual-specificity kinase gene in rice (Oryza sative).","authors":"Han Du, Ying Liang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>We identified and isolated a dual-specificity kinase gene,OsSTY kinase (O. sative serine/threonine/tyrosine kinase) gene, from rice. OsSTY kinase gene encoded a protein of 417 amino acids with calculated molecular weight 45926 Da and isoelectric point 7.689. OsSTY kinase is involved in the multiple stresses signaling pathway. Low- and high-temperature (4 degrees C and 37 degrees C) stresses significantly induce the expression of OsSTY kinase. The kinase is also up-regulated upon wounding (by cut), salicylic acid (SA) and ethophon (ET). Moreover,ectopic expression of OsSTY kinase gene in yeast Saccharomyces cerevisiae ste7/ste7 mutant partially suppressed the pseudohyphal development defect of the strain under the condition of nitrogen starvation. Ste7 is a serine/threonine/tyrosine kinase of Saccharomyces cerevisiae, which shares 32% identity and 50% similarity with OsSTY kinase in the kinase domains. This finding suggested that OsSTY kinase could correct, at least partially, the defect of ste7/ste7 mutant.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Yi chuan xue bao = Acta genetica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
We identified and isolated a dual-specificity kinase gene,OsSTY kinase (O. sative serine/threonine/tyrosine kinase) gene, from rice. OsSTY kinase gene encoded a protein of 417 amino acids with calculated molecular weight 45926 Da and isoelectric point 7.689. OsSTY kinase is involved in the multiple stresses signaling pathway. Low- and high-temperature (4 degrees C and 37 degrees C) stresses significantly induce the expression of OsSTY kinase. The kinase is also up-regulated upon wounding (by cut), salicylic acid (SA) and ethophon (ET). Moreover,ectopic expression of OsSTY kinase gene in yeast Saccharomyces cerevisiae ste7/ste7 mutant partially suppressed the pseudohyphal development defect of the strain under the condition of nitrogen starvation. Ste7 is a serine/threonine/tyrosine kinase of Saccharomyces cerevisiae, which shares 32% identity and 50% similarity with OsSTY kinase in the kinase domains. This finding suggested that OsSTY kinase could correct, at least partially, the defect of ste7/ste7 mutant.