Cloning and characterization of a dual-specificity kinase gene in rice (Oryza sative).

Han Du, Ying Liang
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Abstract

We identified and isolated a dual-specificity kinase gene,OsSTY kinase (O. sative serine/threonine/tyrosine kinase) gene, from rice. OsSTY kinase gene encoded a protein of 417 amino acids with calculated molecular weight 45926 Da and isoelectric point 7.689. OsSTY kinase is involved in the multiple stresses signaling pathway. Low- and high-temperature (4 degrees C and 37 degrees C) stresses significantly induce the expression of OsSTY kinase. The kinase is also up-regulated upon wounding (by cut), salicylic acid (SA) and ethophon (ET). Moreover,ectopic expression of OsSTY kinase gene in yeast Saccharomyces cerevisiae ste7/ste7 mutant partially suppressed the pseudohyphal development defect of the strain under the condition of nitrogen starvation. Ste7 is a serine/threonine/tyrosine kinase of Saccharomyces cerevisiae, which shares 32% identity and 50% similarity with OsSTY kinase in the kinase domains. This finding suggested that OsSTY kinase could correct, at least partially, the defect of ste7/ste7 mutant.

水稻(Oryza satiative)双特异性激酶基因的克隆与鉴定。
我们从水稻中鉴定并分离到了一个双特异性激酶基因,OsSTY激酶(O. sys /苏氨酸/酪氨酸激酶)基因。OsSTY激酶基因编码一个417个氨基酸的蛋白,计算分子量为45926 Da,等电点为7.689。OsSTY激酶参与多种应激信号通路。低温和高温(4℃和37℃)胁迫显著诱导OsSTY激酶的表达。该激酶在受伤(通过切割)、水杨酸(SA)和硫磷(ET)时也上调。此外,异位表达OsSTY激酶基因的酿酒酵母ste7/ste7突变体部分抑制了该菌株在氮饥饿条件下的假菌丝发育缺陷。Ste7是酿酒酵母的一种丝氨酸/苏氨酸/酪氨酸激酶,在激酶结构域上与OsSTY激酶有32%的同源性和50%的相似性。这一发现表明OsSTY激酶至少可以部分纠正ste7/ste7突变体的缺陷。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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