Gas-phase chemistry of the negative ions of fully-protected peptides by high-resolution electrospray ionization tandem mass spectrometry.

Anna Napoli, Leonardo Di Donna, Giovanni Sindona, Elena Urso
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引用次数: 1

Abstract

Fully-protected C-terminal free peptides can be conveniently analyzed by high-resolution electrospray tandem mass spectrometry (ESI-MS/MS) in a quadrupole quadrupole time-of-flight tandem hybrid mass spectrometer, operated in the negative (-) ionizaionization mode. The unusual choice of negative ions in mass spectrometry applications to peptide analysis was needed to obtain exhaustive sequence and structural data. The low-energy collision-induced dissociation (CID) experiments provided, in fact, tandem mass spectra displaying highly diagnostic fragments with a good signal-to-noise ratio. The method is applied to segments of porcine calcitonin (Cal), Cal (1016, 1), Cal (1724, 2) and Cal (2528, 3) whose [M H]- deprotonated molecular ions provided low-energy CID mass spectra which allow the evaluation either of the primary structure of the peptide and of the location of the side-chain protective groups. ESI (+) MS can be conveniently used, in the high resolution mode, to achieve precise information on the elemental composition of the examined peptides.

高分辨率电喷雾串联质谱法研究全保护肽负离子的气相化学。
在负(-)电离模式下,利用高分辨率电喷雾串联质谱(ESI-MS/MS),可以方便地分析完全保护的c端游离肽。在质谱法应用于肽分析中,不寻常的负离子选择需要获得详尽的序列和结构数据。事实上,低能碰撞诱导解离(CID)实验提供了串联质谱,显示出具有良好信噪比的高诊断片段。该方法应用于猪降钙素(Cal), Cal (1016,1), Cal(1724,2)和Cal(2528,3)的片段,其[M H]-去质子化分子离子提供了低能CID质谱,可以评估肽的初级结构和侧链保护基团的位置。ESI(+)质谱可以方便地使用,在高分辨率模式下,获得被检测肽的元素组成的精确信息。
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