The Pseudomonas aeruginosa PAAR2 cluster encodes a putative VRR-NUC domain-containing effector.

IF 5.5 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
FEBS Journal Pub Date : 2021-10-01 Epub Date: 2021-04-25 DOI:10.1111/febs.15870
Shuangyue Wang, Zhi Geng, Heng Zhang, Zhun She, Yuhui Dong
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引用次数: 6

Abstract

The bacterial type VI secretion system (T6SS) secretes many toxic effectors to gain advantage in inter-bacterial competition and for eukaryotic host infection. The cognate immunity proteins of these effectors protect bacteria from the virulence of their own effectors. The T6SS injects its inner-needle Hcp tube, the sharpening tip complex -consisting of VgrG and proline-alanine-alanine-arginine repeats (PAAR) proteins- and toxic effectors into neighboring cells. Its functions are largely determined by the activities of its delivered effectors. Five PAAR proteins were found in the Pseudomonas aeruginosa PAO1 genome with three of them shown to facilitate the delivery of various effectors. Here, we report a putative virus-type replication-repair nuclease domain-containing effector TseV encoded by the least investigated P. aeruginosa PAAR2 cluster. The crystal structure of its putative cognate effector TsiV is presented at 1.6 Å resolution. Through structure and sequence comparisons, we propose TseV-TsiV to be a putative novel effector-immunity (E-I) pair and we discuss the roles of other PAAR2 cluster encoded proteins.

铜绿假单胞菌PAAR2簇编码一个假定的含VRR-NUC结构域的效应物。
细菌VI型分泌系统(T6SS)分泌许多毒性效应物,以在细菌间竞争和真核宿主感染中获得优势。这些效应器的同源免疫蛋白保护细菌免受其自身效应器的毒力。T6SS将其内部针Hcp管、尖尖复合物(由VgrG和脯氨酸-丙氨酸-丙氨酸-精氨酸重复(PAAR)蛋白组成)和毒性效应器注射到邻近细胞中。它的功能在很大程度上取决于它所传递的效应器的活动。在铜绿假单胞菌PAO1基因组中发现了5种PAAR蛋白,其中3种可促进各种效应物的传递。在这里,我们报道了一种假定的病毒型复制修复核酸酶结构域效应物TseV,该效应物由研究最少的铜绿假单胞菌PAAR2簇编码。其假定的同源效应TsiV的晶体结构以1.6 Å分辨率呈现。通过结构和序列比较,我们提出TseV-TsiV可能是一个新的效应免疫(E-I)对,并讨论了其他PAAR2簇编码蛋白的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
FEBS Journal
FEBS Journal 生物-生化与分子生物学
CiteScore
11.70
自引率
1.90%
发文量
375
审稿时长
1 months
期刊介绍: The FEBS Journal is an international journal devoted to the rapid publication of full-length papers covering a wide range of topics in any area of the molecular life sciences. The criteria for acceptance are originality and high quality research, which will provide novel perspectives in a specific area of research, and will be of interest to our broad readership. The journal does not accept papers that describe the expression of specific genes and proteins or test the effect of a drug or reagent, without presenting any biological significance. Papers describing bioinformatics, modelling or structural studies of specific systems or molecules should include experimental data.
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