Immunohistochemical analysis of ghrelin expression in various types of adrenal tumors.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-01-01 Epub Date: 2021-04-09 DOI:10.5603/FHC.a2021.0009

Hanna Komarowska, Agnieszka Malinska, Zhanat Komekbai, Barbara Brominska, Katarzyna Bednarek-Rajewska, Marek Ruchala, Marcin Rucinski

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引用次数: 3

Abstract

INTRODUCTION Ghrelin, originally isolated from the endocrine cells of the gastric mucosa, is also expressed in many peripheral tissues, including normal adrenals and adrenocortical tumors. It was shown that ghrelin stimulates proliferation and inhibits apoptosis of adrenocortical cells. In the current study, we compared ghrelin expression at the protein level in various adrenal tumors. We analyzed whether immunoreactive ghrelin could be considered as a potential marker for different types of adrenal tumors. MATERIAL AND METHODS Study was carried out on 200 adrenal specimens arranged on microscope slide in tissue microarray format. We performed standardized immunohistochemical reactions with semiquantitative reaction intensity measurements. RESULTS At the protein level, the expression of ghrelin was significantly reduced in adrenocortical adenocarcinoma in relation to the control group and pheochromocytoma as well as cancer-adjacent normal adrenal tissue. In contrast, a relatively high ghrelin expression was found in pheochromocytoma compared to all analyzed groups, with the exception of cancer-adjacent normal adrenal tissue. CONCLUSIONS The ghrelin expression profile at the protein level may be associated with the type of adrenal tumors. In this context, our results suggest that adrenal immunoreactive ghrelin may be considered as a sensitive and specific marker for differentiating adrenocortical carcinoma from adrenocortical adenoma and pheochromocytoma.

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不同类型肾上腺肿瘤中胃饥饿素表达的免疫组织化学分析。

胃饥饿素最初是从胃粘膜的内分泌细胞中分离出来的，在许多外周组织中也有表达，包括正常肾上腺和肾上腺皮质肿瘤。结果表明，胃饥饿素刺激肾上腺皮质细胞增殖，抑制细胞凋亡。在本研究中，我们比较了不同肾上腺肿瘤中ghrelin蛋白水平的表达。我们分析了免疫反应性胃饥饿素是否可以被认为是不同类型肾上腺肿瘤的潜在标志物。材料与方法:以组织芯片格式排列于载玻片上的200例肾上腺标本进行研究。我们用半定量的反应强度测量进行标准化的免疫组织化学反应。结果:在蛋白水平上，肾上腺皮质腺癌中ghrelin的表达较对照组和嗜铬细胞瘤及癌旁正常肾上腺组织明显降低。相比之下，除了癌旁正常肾上腺组织外，嗜铬细胞瘤中ghrelin的表达相对较高。结论:在蛋白水平上ghrelin的表达谱可能与肾上腺肿瘤的类型有关。在这种情况下，我们的研究结果表明，肾上腺免疫反应性饥饿素可能被认为是区分肾上腺皮质癌与肾上腺皮质腺瘤和嗜铬细胞瘤的敏感和特异性标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.