Response to 'Comment on "Evaluation of Micronucleus in Exfoliated Human Buccal Epithelium Cells among E-Waste Exposed Residents in Payatas, Philippines"'.

IF 2.4 Q1 Medicine
Journal of Health and Pollution Pub Date : 2021-02-25 eCollection Date: 2021-03-01 DOI:10.5696/2156-9614-11.29.210310
Julie S Berame, Zeba F Alam, Frosyl Miguel, Liz Noguera, Aris Lapada
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引用次数: 0

Abstract

We express our appreciation of the response to our micronucleus paper1 and are glad that our research findings are relevant to other findings on e-waste issues from the plethora of literature that we have read. We understand that there are confounding factors to consider when it comes to micronucleus assays. Based on our reading of other related studies, the use of buccal micronucleus assay for biomonitoring of e-waste workers is definitely possible because the micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage (as also experienced among e-waste workers) in humans. The MN assay in buccal cells has been used since the 1980’s to demonstrate the cytogenetic effects of environmental and occupational exposures, lifestyle factors, dietary deficiencies, and different diseases, but important knowledge gaps remain about the characteristics of micronuclei and other nuclear abnormalities, with the basic biology explaining the appearance of various cell types in buccal mucosa samples and effects of diverse staining procedures and scoring criteria in laboratories around the world. With these uncertainties, the human micronucleus project (HUMN)2 has initiated a new international validation project for the buccal cell MN assay. They advised that future research should explore sources of variability in the assay (e.g., between laboratories and scorers, as well as interand intra-individual differences in subjects), and resolve key technical issues, such as the method of buccal cell staining, optimal criteria for classification of normal and degenerated cells and for scoring micronuclei and other abnormalities. The harmonization and standardization of the buccal MN assay will allow more reliable comparison of data among human populations and laboratories, evaluation of the assay's performance, and consolidation of its world-wide use for biomonitoring of DNA damage.3
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来源期刊
Journal of Health and Pollution
Journal of Health and Pollution Medicine-Public Health, Environmental and Occupational Health
自引率
0.00%
发文量
0
审稿时长
18 weeks
期刊介绍: The Journal of Health and Pollution (JH&P) was initiated with funding from the European Union and World Bank and continues to be a Platinum Open Access Journal. There are no publication or viewing charges. That is, there are no charges to readers or authors. Upon peer-review and acceptance, all articles are made available online. The high-ranking editorial board is comprised of active members who participate in JH&P submissions and editorial policies. The Journal of Health and Pollution welcomes manuscripts based on original research as well as findings from re-interpretation and examination of existing data. JH&P focuses on point source pollution, related health impacts, environmental control and remediation technology. JH&P also has an interest in ambient and indoor pollution. Pollutants of particular interest include heavy metals, pesticides, radionuclides, dioxins, polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs), air particulates (PM10 and PM2.5), and other severe and persistent toxins. JH&P emphasizes work relating directly to low and middle-income countries, however relevant work relating to high-income countries will be considered on a case-by-case basis.
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