Transfer of mitochondria and endosomes between cells by gap junction internalization.

IF 3.6 3区生物学 Q3 CELL BIOLOGY

Traffic Pub Date : 2021-06-01 Epub Date: 2021-04-14 DOI:10.1111/tra.12786

Rachael P Norris

引用次数: 22

Abstract

Intercellular organelle transfer has been documented in several cell types and has been proposed to be important for cell-cell communication and cellular repair. However, the mechanisms by which organelle transfer occurs are uncertain. Recent studies indicate that the gap junction protein, connexin 43 (Cx43), is required for mitochondrial transfer but its specific role is unknown. Using three-dimensional electron microscopy and immunogold labeling of Cx43, this report shows that whole organelles including mitochondria and endosomes are incorporated into double-membrane vesicles, called connexosomes or annular gap junctions, that form as a result of gap junction internalization. These findings demonstrate a novel mechanism for intercellular organelle transfer mediated by Cx43 gap junctions.

查看原文本刊更多论文

通过间隙连接内化在细胞间传递线粒体和核内体。

细胞间细胞器转移已在几种细胞类型中得到证实，并被认为对细胞间通讯和细胞修复非常重要。然而，细胞器转移发生的机制尚不确定。最近的研究表明，间隙连接蛋白连接蛋白43 (Cx43)是线粒体转移所必需的，但其具体作用尚不清楚。利用三维电子显微镜和Cx43的免疫金标记，本报告显示包括线粒体和核内体在内的整个细胞器被纳入双膜囊泡，称为连接体或环形间隙连接，这是由于间隙连接内化而形成的。这些发现证明了一种由Cx43间隙连接介导的细胞器间转移的新机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Traffic 生物-细胞生物学

CiteScore

8.10

自引率

2.20%

发文量

审稿时长

2 months

期刊介绍： Traffic encourages and facilitates the publication of papers in any field relating to intracellular transport in health and disease. Traffic papers span disciplines such as developmental biology, neuroscience, innate and adaptive immunity, epithelial cell biology, intracellular pathogens and host-pathogen interactions, among others using any eukaryotic model system. Areas of particular interest include protein, nucleic acid and lipid traffic, molecular motors, intracellular pathogens, intracellular proteolysis, nuclear import and export, cytokinesis and the cell cycle, the interface between signaling and trafficking or localization, protein translocation, the cell biology of adaptive an innate immunity, organelle biogenesis, metabolism, cell polarity and organization, and organelle movement. All aspects of the structural, molecular biology, biochemistry, genetics, morphology, intracellular signaling and relationship to hereditary or infectious diseases will be covered. Manuscripts must provide a clear conceptual or mechanistic advance. The editors will reject papers that require major changes, including addition of significant experimental data or other significant revision. Traffic will consider manuscripts of any length, but encourages authors to limit their papers to 16 typeset pages or less.