Insight into the effects of electrochemical factors on host-guest interaction induced signature events in a biological nanopore

IF 2.7

Nanotechnology and Precision Engineering Pub Date : 2020-03-01 DOI:10.1016/j.npe.2019.12.001

Xiaojun Wei , Zehui Zhang , Xiaoqin Wang , Brian Lenhart , Roberto Gambarini , Jonathan Gray , Chang Liu

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引用次数: 12

Abstract

The signature events caused by host-guest interactions in the nanopore system can be used as a novel and characteristic signal in quantitative detection and analysis of various molecules. However, the effect of several electrochemical factors on the host-guest interactions in nanopore still remains unknown. Here, we systematically studied host-guest interactions, especially oscillation of DNA-azide adamantane@cucurbit[6] in α-Hemolysin nanopore under varying pH, concentration of electrolytes and counterions (Li⁺, Na⁺, K⁺). Our results indicate correlations between the change of pH and the duration of the oscillation signal. In addition, the asymmetric electrolyte concentration and the charge of the counterions affects the frequency of signature events in oscillation signals, and even the integrity of the protein nanopore. This study provides insight into the design of a future biosensing platform based on signature oscillation signals of the host-guest interaction within a nanopore.

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深入了解电化学因素对生物纳米孔中主客体相互作用诱导的特征事件的影响

纳米孔系统中主客体相互作用引起的特征事件可以作为一种新的特征信号用于各种分子的定量检测和分析。然而，几种电化学因素对纳米孔中主客体相互作用的影响尚不清楚。在这里，我们系统地研究了主-guest相互作用，特别是dna -叠氮化物adamantane@cucurbit[6]在不同pH、电解质浓度和反离子(Li+， Na+， K+)下α-溶血素纳米孔中的振荡。我们的结果表明pH值的变化与振荡信号的持续时间之间存在相关性。此外，不对称的电解质浓度和反离子的电荷影响振荡信号中特征事件的频率，甚至影响蛋白质纳米孔的完整性。这项研究为基于纳米孔内主客体相互作用的特征振荡信号的未来生物传感平台的设计提供了见解。

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Nanotechnology and Precision Engineering

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