Chemical Effects of Kainic Acid Injection into the Rat Superior Olivary Region.

Abstract

Kainic acid injections have been used to destroy neuron somata in particular regions without damaging fiber tracts. We injected a solution of kainic acid into the region of the rat superior olivary complex in an effort to destroy its cholinergic projections to the cochlea and cochlear nucleus, which derive especially from the lateral superior olivary nucleus and ventral nucleus of the trapezoid body. In the lateral superior olivary nucleus, there were relatively small but fairly consistent decreases of choline acetyltransferase (ChAT) activity, larger decreases of acetylcholinesterase (AChE) activity, and consistent decreases of malate dehydrogenase activity, as a marker for oxidative metabolism. Other superior olivary regions were less affected by the kainic acid injections, but most showed overall significant decreases of AChE activity. Our results suggest that the cholinergic neurons giving rise to the centrifugal pathways to the cochlea and cochlear nucleus are more resistant to the effects of kainic acid than are those that receive major ascending input from the cochlear nucleus and project to higher levels of the auditory system. Comparison with published anatomical studies suggests that this resistance to the effects of kainic acid is related to relatively little glutamatergic input to the somata and proximal dendrites of these neurons. We also found a consistent approximately 16 % decrease of ChAT activity in the injected-side facial nerve root, which is most easily explained as a small effect of kainic acid on the facial nerve fibers passing through the injection site.