Evaluation of Antimicrobial Effects on Dental Impression Materials and Biofilm Removal by Sodium Dichloroisocyanurate.

IF 0.9 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hiroko Hiramine, Kiyoko Watanabe, Keitaro Inaba, Haruka Sasaki, Nobushiro Hamada
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引用次数: 3

Abstract

Dental materials are inevitably contaminated with oral microorganisms. To prevent transmission of infectious diseases, impressions need to be disinfected. In the present study, we examined the disinfection effects on impression materials and biofilm removal by sodium dichloroisocyanurate (SDIC). Exponentially growing Streptococcus mutans, Escherichia coli, Staphylococcus aureus and Candida albicans, and dental plaque bacteria were suspended in phosphate buffered saline (PBS) and exposed for 1, 5 and 10 min to 1 mL of the 10 ppm, 100 ppm, 1,000 ppm, and 10,000 ppm SDIC solutions. The bactericidal effect was evaluated by colony forming units of each microorganisms. Moreover, the effect of SDIC solution on S. mutans biofilm was examined. Bactericidal effects of SDIC solutions on oral bacteria on dental impression surfaces were assessed and the surface quality of dental casts after immersion in SDIC solution for 30 min was observed under a scanning electron microscope. The number of all bacterial strains, including plaque bacteria, were significantly decreased by SDIC solution treatment in a dose-dependent manner. Significant S. mutans biofilm removing activity of SDIC was observed in 1,000 and 10,000 ppm solution. The number of oral bacteria adhering to the surfaces of impressions markedly decreased following 10-min immersion in the 1,000 ppm SDIC solution. The 30-min immersion of dental impression in the 1,000 ppm SDIC solution did not adversely affect the surface roughness of dental casts. The results indicate that SDIC Solution is useful to deactivate oral bacteria on dental impression.

二氯异氰尿酸钠对牙印模材料的抗菌效果及生物膜去除效果评价。
口腔微生物不可避免地会污染牙科材料。为了防止传染病的传播,印痕需要消毒。在本研究中,我们研究了二氯异氰尿酸钠(SDIC)对印模材料和生物膜去除的消毒效果。将呈指数增长的变形链球菌、大肠杆菌、金黄色葡萄球菌和白色念珠菌以及牙菌斑细菌悬浮在磷酸盐缓冲盐水(PBS)中,分别在1ml的10ppm、100ppm、1000ppm和10000ppm的SDIC溶液中暴露1,5和10min。用菌落形成单位评价各微生物的杀菌效果。此外,还考察了SDIC溶液对变形链球菌生物膜的影响。观察SDIC溶液对口腔印模表面细菌的杀菌效果,并在扫描电镜下观察SDIC溶液浸泡30 min后牙模的表面质量。SDIC溶液处理后,包括菌斑细菌在内的所有细菌菌株数量均呈剂量依赖性显著减少。SDIC在1,000和10,000 ppm溶液中具有显著的变形链球菌生物膜去除活性。在1,000 ppm的SDIC溶液中浸泡10分钟后,粘附在印模表面的口腔细菌数量明显减少。牙模在1000 ppm的SDIC溶液中浸泡30分钟,对牙模的表面粗糙度没有不良影响。结果表明,SDIC溶液对牙印模上的口腔细菌有一定的灭活作用。
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来源期刊
Biocontrol science
Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
2.60
自引率
8.30%
发文量
21
审稿时长
>12 weeks
期刊介绍: The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.
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