Identification of Phytoconstituents of Tragia Involucrata leaf Extracts and Evaluate their Correlation with Anti-inflammatory & Antioxidant Properties.

Q2 Medicine

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pub Date : 2021-01-01 DOI:10.2174/1871523020666210126144506

Vinodhini Velu, Swagata Banerjee, Vidya Radhakrishnan, Gaurav Gupta, Dinesh Kumar Chellappan, Neeraj Kumar Fuloria, Shivkanya Fuloria, Meenu Mehta, Kamal Dua, Himaja Malipeddi

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引用次数: 5

Abstract

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts.

Materials and methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using Soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H₂O₂ method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be a major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract.

Results and discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28-epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine- 1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87%) and H₂O₂ method (73.58%) at 200 μg mL^-1. Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study.

Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent anti- inflammatory property.

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天葵叶提取物的成分鉴定及其与抗炎、抗氧化作用的相关性研究。

目的:采用气相色谱-质谱法对其成分进行分析，评价其抗氧化和抗炎活性。材料和方法:用索氏装置用石油醚、乙酸乙酯和水依次提取。采用气相色谱-质谱法对所鉴定化合物的抗炎性能进行了评价。采用DPPH和H2O2法研究其抗氧化活性，采用HRBC膜稳定法研究其抗炎活性。石油醚提取物以萜类化合物为主，乙酸乙酯提取物以酚类和黄酮类化合物为主。结果与讨论:经GC-MS分析，提取液中含有角鲨烯、19β、28-环氧leana -3-醇和2-图-丁基-5-氯甲基-3-甲基-4-氧咪唑烷- 1-羧酸6个主要分子。乙酸乙酯提取物在200 μg mL-1时具有显著的抗氧化活性(P2O2法为73.58%)。在体外抗炎活性研究中，观察到石油醚提取物增强了膜稳定性。萜类化合物含量与抗炎活性的相关性(0.971)和对接研究表明，萜类化合物含量与抗炎活性之间存在较强的相关关系。结论:上述结果证明天秤座含有丰富的萜类化合物，具有良好的抗炎作用。

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来源期刊

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.30

自引率

0.00%

发文量

期刊介绍： Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-inflammatory & anti-allergy agents. Publishing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.