Monoclonal antibodies against Enterocytozoon bieneusi of human origin.

Abhineet S Sheoran, Xiaochuan Feng, Inderpal Singh, Susan Chapman-Bonofiglio, Sabrina Kitaka, Joel Hanawalt, John Nunnari, Keith Mansfield, James K Tumwine, Saul Tzipori
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引用次数: 22

Abstract

Enterocytozoon bieneusi is clinically the most significant among the microsporidia infecting humans, causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. The lack of immune reagents is largely due to the absence of methods for laboratory propagation of E. bieneusi. We recently described a procedure for the concentration and purification of spores from diarrheic stool of infected humans. Purified spores were used to immunize mice for production and screening of monoclonal antibodies (MAbs) against E. bieneusi. The eight immunoglobulin M MAbs generated and fully characterized did not cross-react with other human microsporidia or with other microorganisms normally present in stool. One of the MAbs, 2G4, reacted with E. bieneusi spores in stools from monkeys and humans, without background fluorescence, which makes it an ideal diagnostic reagent. It also recognizes intracellular stages of the parasite and will be suitable for determining tissue distribution of E. bieneusi in infected hosts. At least two immunodominant antigens of E. bieneusi of 33,000 and 35,000 Da exist, which were recognized by rabbit and mouse antisera. The availability of MAbs against E. bieneusi will simplify considerably the diagnosis of this infection in humans and will provide tools for epidemiologic investigations regarding the true prevalence of the infection in various human and mammalian populations and the environmental sources of infection.

抗人源双胞虫单克隆抗体。
bieneusenterocytozoon bieneusi是临床上最重要的感染人类的微孢子虫,在人类免疫缺陷病毒/艾滋病患者中引起慢性腹泻、消瘦和胆管炎。免疫试剂的缺乏主要是由于缺乏实验室繁殖的方法。我们最近描述了一种从被感染的人的腹泻粪便中浓缩和纯化孢子的程序。利用纯化的孢子免疫小鼠,制备和筛选抗布氏单抗(mab)。制备的八种免疫球蛋白M单抗与其他人类微孢子虫或粪便中通常存在的其他微生物没有交叉反应。其中一种单克隆抗体2G4与猴子和人类粪便中的比氏埃希菌孢子发生反应,没有背景荧光,这使其成为一种理想的诊断试剂。它还可以识别寄生虫的细胞内阶段,并将适用于确定受感染宿主的组织分布。至少存在33000和35000 Da的两种免疫优势抗原,可被兔和小鼠抗血清识别。针对布氏埃氏体的单克隆抗体的可用性将大大简化这种人类感染的诊断,并将为流行病学调查提供工具,了解感染在各种人类和哺乳动物群体中的真实流行情况以及感染的环境来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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