Yoshiki Yagita , Isaac Barjis , Michael Hecht , Helene Bach , David A. Feldheim , Frank Scalia
{"title":"Partial nucleotide sequences and expression patterns of frog (Rana pipiens) ephrin-A2 and ephrin-A5 mRNA","authors":"Yoshiki Yagita , Isaac Barjis , Michael Hecht , Helene Bach , David A. Feldheim , Frank Scalia","doi":"10.1016/j.devbrainres.2005.06.016","DOIUrl":null,"url":null,"abstract":"<div><p>We have generated 362 bp and 547 bp partial sequences for <span><em>Rana pipiens</em></span><span><span> ephrin-A2 and ephrin-A5 mRNA, respectively. Translation homologies for the comparable segments of cDNA of chicken, mouse and human are 90.8, 86.9 and 84.4% for the ephrin-A2 sequence and 85.7, 85.0 and 85.0% for the ephrin-A5 sequence. Digoxigenin-labeled riboprobes<span><span> were prepared and applied by means of in situ hybridization to whole-mounts of the brains of mature adults and expression patterns in </span>tadpoles were also explored. The RNA probes revealed similar posterior (high) to anterior (low) expression gradients in the adult </span></span>tectum, demonstrating that both ephrin-As are expressed in the adult Ranid frog tectum. Only the ephrin-A2 probe was tested on tadpole brain, yielding an appropriately graded expression pattern similar to the adult.</span></p></div>","PeriodicalId":100369,"journal":{"name":"Developmental Brain Research","volume":"159 1","pages":"Pages 72-77"},"PeriodicalIF":0.0000,"publicationDate":"2005-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.devbrainres.2005.06.016","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental Brain Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165380605001811","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
We have generated 362 bp and 547 bp partial sequences for Rana pipiens ephrin-A2 and ephrin-A5 mRNA, respectively. Translation homologies for the comparable segments of cDNA of chicken, mouse and human are 90.8, 86.9 and 84.4% for the ephrin-A2 sequence and 85.7, 85.0 and 85.0% for the ephrin-A5 sequence. Digoxigenin-labeled riboprobes were prepared and applied by means of in situ hybridization to whole-mounts of the brains of mature adults and expression patterns in tadpoles were also explored. The RNA probes revealed similar posterior (high) to anterior (low) expression gradients in the adult tectum, demonstrating that both ephrin-As are expressed in the adult Ranid frog tectum. Only the ephrin-A2 probe was tested on tadpole brain, yielding an appropriately graded expression pattern similar to the adult.
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