Role of gene expression profiling for diagnosing acute leukemias.

Wolfgang Kern, Alexander Kohlmann, Susanne Schnittger, Claudia Schoch, Torsten Haferlach
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Abstract

Cytomorphology and cytochemistry in combination with multiparameter immunophenotyping today are the standard methods for establishing the diagnosis of acute leukemias. In addition, cytogenetics, fluorescence in situ hybridization, and polymerase chain reaction based assays provide important information regarding biologically defined and prognostically relevant subgroups and allow a comprehensive diagnosis of well defined subentities. With regard to the clinical setting a better understanding of the clinical course of distinct biologically defined disease subtypes is needed to select disease-specific therapeutic approaches. Paralleling the increase in knowledge on deregulated pathways in leukemia the development of new therapeutics is accelerated and therefore requires a detailed and comprehensive diagnostic tool. Revealing and quantifying the expression status of many ten thousands of genes in a single analysis the microarray technology holds this potential to become an essential tool for the molecular classification of leukemias. It may therefore be used as a routine method for diagnostic purposes in the near future. Furthermore, it is anticipated that new biologically defined and clinically relevant subtypes of leukemia will be identified based on gene expression profiling. This method may therefore guide therapeutic decisions.

基因表达谱在急性白血病诊断中的作用。
目前,细胞形态学和细胞化学结合多参数免疫表型是建立急性白血病诊断的标准方法。此外,细胞遗传学、荧光原位杂交和基于聚合酶链反应的检测提供了关于生物学定义和预后相关亚群的重要信息,并允许对定义明确的亚实体进行全面诊断。关于临床环境,需要更好地了解不同生物学定义的疾病亚型的临床病程,以选择疾病特异性治疗方法。随着对白血病中不受管制的途径的认识的增加,新疗法的发展也在加速,因此需要一种详细和全面的诊断工具。微阵列技术在一次分析中揭示和量化成千上万个基因的表达状态,有可能成为白血病分子分类的重要工具。因此,在不久的将来,它可能被用作诊断目的的常规方法。此外,预计新的生物学定义和临床相关的白血病亚型将基于基因表达谱被确定。因此,这种方法可以指导治疗决策。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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