[Genome constitution of Agropyron elongatum 4x by biochemical and SSR markers].

Na Li, Xian-Ping Wang, Shuang-He Cao, Xiang-Qi Zhang
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Abstract

We analyzed the electrophoritic patterns of 16 kinds of isozyme and three kinds of storage protein in Agropyron elongatum 2x and Ag. elongatum 4x by isoelectric focusing (IEF) and SDS-PAGE, respectively. The results showed three types of electrophoritic patterns. In the first type, the zymogram phenotypes are identical between Ag. elongatum 2x and Ag. elongatum 4x. Two kinds of isozyme belong to this type, which accounts for 10.5% of all the biochemical markers analyzed. In the second type, Ag. elongatum 4x showed a phenotype which is made up of combinations of the all of Ag. elongatum 2x bands and the specific bands. Ten kinds of isozyme and three kinds of storage protein fall into this class, which is the most part and accounts for 63.2% of all the markers analyzed. In the third type, Ag. elongatum 2x and Ag. elongatum 4x showed some identical bands and their own specific bands. Five kinds of isozyme were classified to this group,which accounts for 26.3% of all the markers analyzed. The results above suggested that Ag. elongatum 4x is a allotetraploid composed of one genome originated from Ag. elongatum 2x and another unknown genome which is apparently distinct from the St, J and N genomes of relative species. Otherwise, SSR primers were used to analyze the relation of Ag. elongatum 2x and Ag. elongatum 4x. The amplification results of most primers showed that Ag. elongatum 2x and Ag. elongatum 4x have some identical bands and Ag. elongatum 4x have some specific bands. This result validated the conclusion from biochemical marker analyses that Ag. elongatum 4x is a allotetraploid.

[通过生化标记和SSR标记对Agropyron elongatum 4x基因组构成的分析]。
分析了长柄Agropyron 2x和Ag中16种同工酶和3种贮藏蛋白的电泳模式。通过等电聚焦(IEF)和SDS-PAGE分别对伸长体4x进行了分析。结果显示出三种类型的电泳模式。在第一种类型,酶谱表型是相同的Ag。伸长体2x和Ag。elongatum 4 x。这类同工酶有两种,占分析的所有生化标记物的10.5%。在第二种类型中,Ag。长形体4x表现出由所有Ag组合而成的表型。细长体2x波段和特定波段。10种同工酶和3种储存蛋白属于这一类,是最多的,占所有分析标记的63.2%。在第三类中,Ag。伸长体2x和Ag。伸长体4x显示出一些相同的条带和自己的特定条带。这类同工酶有5种,占所有分析标记的26.3%。上述结果表明,Ag。拉长体4x是由一个基因组组成的异源四倍体。长形体2x和另一个未知的基因组,明显不同于相关物种的St, J和N基因组。另外,利用SSR引物分析了Ag的亲缘关系。伸长体2x和Ag。elongatum 4 x。多数引物的扩增结果表明,Ag。伸长体2x和Ag。长形体4x有一些相同的带和Ag。伸长体4x有一些特殊的条带。这一结果验证了生化标记分析的结论。拉长体4x是异源四倍体。
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