Jianjiang Zhou, Manling Chen, Qunzhou Zhang, Jiankun Hu, Wenling Wang
{"title":"Human gastric tissues simultaneously express the classical and alternative splicing cholecystokinin-B/gastrin receptors.","authors":"Jianjiang Zhou, Manling Chen, Qunzhou Zhang, Jiankun Hu, Wenling Wang","doi":"10.3109/10606820490926179","DOIUrl":null,"url":null,"abstract":"<p><p>To explore whether cholecystokinin-B/gastrin receptor (CCKBRwt) gene and its alternative splicing variant preserving intron 4 (CCKBRi4sv) are expressed in human gastric carcinoma cell line and tissue, we detect mRNA expression of CCKBRwt and CCKBRi4sv in 30 gastric carcinoma and their corresponding normal tissues, 10 gastritis, and 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells by RT-PCR and sequencing. The results revealed that human normal, inflammatory, and malignant gastric tissues simultaneously expressed the classical and alternative splicing cholecystokinin-B/gastrin receptor genes. The alternative splicing variant contains the intron 4 of cholecystokinin-B/gastrin receptor gene.</p>","PeriodicalId":20928,"journal":{"name":"Receptors & channels","volume":"10 5-6","pages":"185-8"},"PeriodicalIF":0.0000,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10606820490926179","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Receptors & channels","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10606820490926179","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
To explore whether cholecystokinin-B/gastrin receptor (CCKBRwt) gene and its alternative splicing variant preserving intron 4 (CCKBRi4sv) are expressed in human gastric carcinoma cell line and tissue, we detect mRNA expression of CCKBRwt and CCKBRi4sv in 30 gastric carcinoma and their corresponding normal tissues, 10 gastritis, and 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells by RT-PCR and sequencing. The results revealed that human normal, inflammatory, and malignant gastric tissues simultaneously expressed the classical and alternative splicing cholecystokinin-B/gastrin receptor genes. The alternative splicing variant contains the intron 4 of cholecystokinin-B/gastrin receptor gene.
为了探讨胆囊收缩素- b /胃泌素受体(CCKBRwt)基因及其选择性剪接变体保存子4 (CCKBRi4sv)是否在人胃癌细胞系和组织中表达，我们采用RT-PCR和测序的方法检测了30例胃癌及其相应的正常组织、10例胃炎和2例尸检正常胃标本以及胃癌细胞系SGC-7901细胞中CCKBRwt和CCKBRi4sv的mRNA表达。结果显示，正常、炎症和恶性胃组织同时表达经典剪接和选择性剪接的胆囊收缩素- b /胃泌素受体基因。备选剪接变体包含胆囊收缩素- b /胃泌素受体基因的内含子4。
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
