Effects of Azadirachta indica and Melia azedarach (Meliaceae) extracts from leaves on Trypanosoma cruzi growth and ultrastructure.

A Yanes, H J Finol, M Hasegawa
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引用次数: 0

Abstract

The chloroformic extracts from dried fresh leaves ofAzadirachta indica A. Juss. and Melia azedarach L. (Meliaceae) showed marked inhibitory activity on epimastigotes growth of Trypanosoma cruzi, evidenced by 96-wells microtiter plate bioassay and radioactive thymidine incorporation experiment. Each chloroformic extract was separated using silica gel and alumina column. In transmission electron microscopy the bioactive chromatographic fractions caused ultrastructural changes in epimastigotes such as vacuolization probably induced by degeneration of the kinetoplast-mitochondrion complex, organelle degeneration, and cell division disruption. In spectral analysis these bioactive fractions seemed to be composed mainly of fatty acid mixtures.

印楝和苦楝叶提取物对克氏锥虫生长和超微结构的影响。
从印楝干鲜叶中提取氯甲酸提取物。96孔微滴板生物测定和放射性胸腺嘧啶掺入实验表明,苦楝属植物对克氏锥虫的附生毛囊有明显的抑制作用。采用硅胶柱和氧化铝柱分离氯甲酸提取液。在透射电镜下,生物活性色谱组分引起了外生毛囊的超微结构变化,如空泡化,这可能是由着丝体-线粒体复合体的退化、细胞器退化和细胞分裂中断引起的。在光谱分析中,这些生物活性组分似乎主要由脂肪酸混合物组成。
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